Department of Biology and Environmental Sciences, Texas A&M University at Commerce, 2600 S. Neal Street, Commerce, TX 75428, USA.
J Neurosci Methods. 2010 May 30;189(1):1-4. doi: 10.1016/j.jneumeth.2010.02.015. Epub 2010 Feb 19.
Brain development and health depends upon the efficient movement of the cerebrospinal fluid inside of brain ventricles. When disrupted either through mutation, disease, or physiological damage, brain function becomes significantly impaired. Here I present a simple method of following cerebrospinal fluid circulation in Xenopus tadpoles using fluorescent microspheres which can be applied to imaging fluid circulation in any transparent embryo. In particular, cilia may be labeled with these microspheres to study their dynamics and movement patterns in vivo while simultaneously measuring bulk fluid flow. This technique will facilitate the analysis of fluid dynamics in developing embryos and aid in understanding the regulation of cilia dependent fluid flow in vivo.
脑发育和健康依赖于脑室内脑脊液的有效流动。当这种流动因突变、疾病或生理损伤而受到干扰时，脑功能会受到严重损害。在这里，我提出了一种使用荧光微球跟踪非洲爪蟾蝌蚪脑脊液循环的简单方法，该方法可应用于任何透明胚胎的流体循环成像。特别是，可以用这些微球标记纤毛，以在体内研究它们的动力学和运动模式，同时测量整体流体流动。该技术将有助于分析发育中的胚胎中的流体动力学，并有助于理解体内纤毛依赖的流体流动的调节。
