Stimpson D I, Cann J R
Biophys Chem. 1977 Sep;7(2):115-9. doi: 10.1016/0301-4622(77)80004-5.
Transient and equilibrium isoelectric focusing patterns have been computed for pH-dependent conformational transitions in the limits of complete cooperativity and instantaneous chemical equilibration. Transitions induced by the binding of a relatively large number of hydrogen ions by the macromolecule give well resolved bimodal equilibrium patterns, provided that the resulting conformer has the lower isoelectric point. The corresponding transient patterns may be either bimodal or virtually unimodal for practical times of operation depending upon the point of insertion of the sample into the pH gradient and the stoichiometry of the interaction. A macromolecule undergoing sequential transitions can give multimodal isoelectric focussing patterns.
在完全协同性和瞬时化学平衡的极限条件下，已计算出pH依赖性构象转变的瞬态和平衡等电聚焦模式。如果大分子结合相对大量氢离子所诱导的转变产生的构象异构体具有较低的等电点，那么这种转变会产生分辨率良好的双峰平衡模式。根据样品插入pH梯度的位置以及相互作用的化学计量，在实际操作时间内，相应的瞬态模式可能是双峰的，也可能实际上是单峰的。经历连续转变的大分子可以给出多峰等电聚焦模式。
