Faculty of Pharmacy, Tehran University of Medical Sciences, Tehran, Iran.
Nanomedicine. 2010 Oct;6(5):689-97. doi: 10.1016/j.nano.2010.01.011. Epub 2010 Feb 18.
The aim of the present work was to prepare and characterize poly(methyl methacrylate) nanoparticles coated by chitosan-glutathione conjugate so as to encapsulate insoluble anticancer drugs. Nanoparticles were synthesized through radical polymerization of methyl methacrylate initiated by cerium (IV) ammonium nitrate. Paclitaxel (PTX), a model anticancer drug, was encapsulated in nanoparticles with a maximal encapsulation efficiency of 98.27%. These nanoparticles showed sustained in vitro release of the incorporated PTX (75% of the loaded dose was released in 10 days). All nanoparticles had positive charge and were spherical, with a size range of about 130-250 nm. The PTX-loaded nanoparticles showed cytotoxicity for NIH 3T3 and T47D breast carcinoma cells, along with no cytotoxicity for two colon cell lines (HT29, Caco2).
The aim of this work was to prepare and characterize poly(methyl methacrylate) nanoparticles coated by chitosan-glutathione conjugate in an effort to encapsulate Paclitaxel as a model of insoluble anticancer drugs. These nanoparticles showed sustained in vitro drug release.
本工作旨在制备并表征壳聚糖-谷胱甘肽缀合物包覆的聚甲基丙烯酸甲酯纳米粒子，以包裹难溶性抗癌药物作为模型药物。纳米粒子通过硝酸铈(IV)铵引发的甲基丙烯酸甲酯自由基聚合合成。紫杉醇（PTX）是一种模型抗癌药物，其包封效率最高可达 98.27%。这些纳米粒子表现出对所载 PTX 的持续体外释放（10 天内释放了 75%的载药量）。所有纳米粒子均带正电荷，呈球形，粒径约为 130-250nm。载有 PTX 的纳米粒子对 NIH 3T3 和 T47D 乳腺癌细胞具有细胞毒性，而对两种结肠细胞系（HT29、Caco2）没有细胞毒性。
本工作旨在制备并表征壳聚糖-谷胱甘肽缀合物包覆的聚甲基丙烯酸甲酯纳米粒子，以包裹紫杉醇作为难溶性抗癌药物的模型药物。这些纳米粒子显示出持续的体外药物释放。
