School of Natural Sciences, Edith Cowan University, Perth, Western Australia 6027, Australia.
Anal Chim Acta. 2010 Mar 17;663(1):105-8. doi: 10.1016/j.aca.2010.01.043. Epub 2010 Feb 1.
Glucoraphanin, a glucosinolate, is found naturally in plants and is present in relatively high concentrations in broccoli. Glucosinolates have received much attention as studies have indicated that a diet rich in them may provide some protection from certain cancers. A micellar electrokinetic chromatography (MEKC) method using sodium cholate as the micellar phase has been developed to quantify for glucoraphanin in broccoli (seeds and florets) and Brussels sprouts. The glucoraphanin peak elutes just under 5min with a theoretical plate number of 380,000 per metre of capillary. The method is suitable for crude extracts of broccoli and Brussels sprouts. Glucoraphanin in broccoli seeds (1330mg/100g) broccoli florets (89mg/100g) and Brussels sprouts (3mg/100g) was determined and agreed with the data obtained by high performance liquid chromatography. The LODs were 10-100 times below the levels typically found in broccoli seeds (4mg/100g), broccoli florets (0.9mg/100g) and Brussels sprouts (0.1mg/100g).
萝卜硫苷，一种硫代葡萄糖苷，天然存在于植物中，在西兰花中含量相对较高。由于研究表明，富含硫代葡萄糖苷的饮食可能对某些癌症提供一定的保护，因此硫代葡萄糖苷受到了广泛关注。本文建立了一种使用胆酸钠作为胶束相的胶束电动色谱（MEKC）方法，用于定量分析西兰花（种子和花椰菜）和抱子甘蓝中的萝卜硫苷。萝卜硫苷峰在 5 分钟内洗脱，理论塔板数为每米毛细管 380000。该方法适用于西兰花和抱子甘蓝的粗提取物。测定了西兰花种子（1330mg/100g）、西兰花花椰菜（89mg/100g）和抱子甘蓝（3mg/100g）中的萝卜硫苷，与高效液相色谱法获得的数据一致。LODs 比西兰花种子（4mg/100g）、西兰花花椰菜（0.9mg/100g）和抱子甘蓝（0.1mg/100g）中通常发现的水平低 10-100 倍。
