National Research Institute for Food and Nutrition-INRAN, Via Ardeatina 546, 00178, Roma, Italy.
J Mass Spectrom. 2012 Sep;47(9):1198-206. doi: 10.1002/jms.3028.
Glucosinolates are an important class of secondary plant metabolites, possessing health-promoting properties. Young broccoli plants are a very good source of glucosinolates with concentrations several times greater than in mature plants. The aim of our study was to develop a liquid chromatography-mass spectrometry and liquid chromatography/tandem mass spectrometry qualitative and quantitative method for the measure of glucosinolates in broccoli sprouts. The described method provides high sensitivity and specificity, allowing a rapid and simultaneous determination of 14 glucosinolates. The proposed method has been validated for eight glucosinolates: glucobrassicin, glucoraphanin, glucoiberin, glucoerucin, progoitrin, gluconapin, sinigrin and glucocheirolin. The linear range was 1-150 µg ml(-1), the intra-day and inter-day precision values are within 6% and 8% at the lower limit of quantification, while the overall recovery of the eight glucosinolates was 99 ± 9%. This validated method was used successfully for analysis of glucosinolates content of broccoli sprouts grown in different conditions.
硫代葡萄糖苷是一类重要的次生代谢植物产物，具有促进健康的特性。幼嫩的西兰花植株是硫代葡萄糖苷的极好来源，其浓度比成熟植株高几倍。我们研究的目的是开发一种用于测量西兰花芽中硫代葡萄糖苷的液相色谱-质谱和液相色谱/串联质谱定性和定量方法。所描述的方法具有高灵敏度和特异性，允许快速同时测定 14 种硫代葡萄糖苷。该方法已对 8 种硫代葡萄糖苷（葡萄糖硫苷、萝卜硫素、葡萄糖苷、葡萄糖异硫氰酸盐、前芸薹素、葡萄糖苷、黑芥子苷和葡萄糖芥醇）进行了验证。线性范围为 1-150 µg ml(-1)，在定量下限处，日内和日间精密度值在 6%和 8%之间，而 8 种硫代葡萄糖苷的总回收率为 99 ± 9%。该经验证的方法成功地用于分析在不同条件下生长的西兰花芽中的硫代葡萄糖苷含量。
