Department of Otolaryngology-Head and Neck Surgery, College of Medicine, The Catholic University of Korea, Seoul, Korea.
Otolaryngol Head Neck Surg. 2010 Mar;142(3):370-5. doi: 10.1016/j.otohns.2009.11.025. Epub 2010 Jan 22.
Induction of oral tolerance (OT) is known to prevent allergic inflammation in models of asthma. This study investigated the preventive effect of OT and airway remodeling in a mouse model of allergic rhinitis (AR).
An in vivo study using an animal model.
Catholic Research Institutes of Medical Science.
Forty six-week-old, female BALB/c mice were divided into four groups: control, AR, low-dose OT, and high-dose OT. To induce OT, mice were fed ovalbumin (OVA) before sensitization with OVA/aluminum hydroxide, 1 mg for six days in the low-dose OT group and a 25 mg single dose in the high-dose OT group. Mice in the AR group were fed phosphate-buffered saline. After sensitization followed by challenges with OVA during six weeks, nasal behaviors, interleukin (IL)-13 and interferon gamma (IFN-gamma) levels in nasal lavage (NAL) fluids, as well as OVA-specific IgE levels in serum, were measured. The degree of goblet cell hyperplasia and thickness of lamina propria were observed in nasal tissues by periodic acid-Schiff and Masson's trichrome stain. A P value < 0.05 was accepted as statistically significant.
Both OT groups showed a significant decrease in inflammatory cells, IL-13 and IFN-gamma in NAL fluids, as well as OVA-specific IgE levels in serum compared with the AR group. In addition, the degree of goblet cell hyperplasia and thickness of lamina propria were attenuated in both OT groups compared with the AR group. Further, these alterations did not differ significantly between the two OT groups.
These results suggest that OT may effectively reduce allergic inflammation as well as airway remodeling in a mouse model of AR.
诱导口服耐受（OT）已被证实可预防哮喘模型中的过敏炎症。本研究旨在探讨 OT 对变应性鼻炎（AR）小鼠模型的预防作用及其对气道重塑的影响。
体内动物模型研究。
天主教医疗科学研究所。
将 46 周龄雌性 BALB/c 小鼠分为四组：对照组、AR 组、低剂量 OT 组和高剂量 OT 组。为了诱导 OT，在致敏前用卵清蛋白（OVA）喂养小鼠，低剂量 OT 组连续 6 天给予 OVA/氢氧化铝 1mg，高剂量 OT 组给予 OVA/氢氧化铝 25mg 单次剂量。AR 组给予磷酸盐缓冲液。致敏后，用 OVA 进行 6 周攻毒，测量鼻行为、鼻洗液（NAL）中白细胞介素（IL）-13 和干扰素-γ（IFN-γ）水平以及血清中 OVA 特异性 IgE 水平。通过过碘酸希夫（PAS）和 Masson 三色染色观察鼻组织中杯状细胞增生和固有层厚度。P 值<0.05 被认为具有统计学意义。
与 AR 组相比，OT 两组均显示出 NAL 液中炎症细胞、IL-13 和 IFN-γ以及血清中 OVA 特异性 IgE 水平显著降低。此外，与 AR 组相比，OT 两组的杯状细胞增生程度和固有层厚度均减轻。此外，这两种 OT 组之间的这些改变没有显著差异。
这些结果表明，OT 可能有效减轻 AR 小鼠模型中的过敏炎症和气道重塑。
