大肠杆菌必需 GTPase Der 通过 KH 样结构域与 50S 核糖体相互作用。
Interaction of an essential Escherichia coli GTPase, Der, with the 50S ribosome via the KH-like domain.
机构信息
Center for Advanced Biotechnology and Medicine, Department of Biochemistry, Robert Wood Johnson Medical School, University of Medicine and Dentistry of New Jersey, 679 Hoes Lane, Piscataway, NJ 08854, USA.
出版信息
J Bacteriol. 2010 Apr;192(8):2277-83. doi: 10.1128/JB.00045-10. Epub 2010 Feb 19.
Abstract
Der, an essential Escherichia coli tandem GTPase, has been implicated in 50S subunit biogenesis. The rrmJ gene encodes a methyltransferase that modifies the U2552 residue of 23S rRNA, and its deletion causes a severe growth defect. Peculiarly, overexpression of Der suppresses growth impairment. In this study, using an rrmJ-deletion strain, we demonstrated that two GTPase domains of Der regulate its association with 50S subunit via the KH-like domain. We also identified a region of Der that is critical for its specific interaction with 50S subunit.
摘要
Der，一种必需的大肠杆菌串联 GTPase，已被牵连到 50S 亚基生物发生中。rrmJ 基因编码一种甲基转移酶，修饰 23S rRNA 的 U2552 残基，其缺失导致严重的生长缺陷。奇怪的是，Der 的过表达抑制了生长损伤。在这项研究中，我们使用 rrmJ 缺失菌株表明 Der 的两个 GTPase 结构域通过 KH 样结构域调节其与 50S 亚基的结合。我们还确定了 Der 的一个区域对于其与 50S 亚基的特异性相互作用至关重要。
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