法沃氏菌属，新种，一种从中性盐度油污染土壤中分离得到的中度嗜盐细菌。
Sporosalibacterium faouarense gen. nov., sp. nov., a moderately halophilic bacterium isolated from oil-contaminated soil.
机构信息
Laboratoire de Microbiologie, Groupe des Bioprocédés, Institut Pasteur de Tunis, BP 74, Place Pasteur, Belvédère, Tunis 1002, Tunisia.
Microbiologie et Biotechnologie des Environnements Chauds, UMR D180, IRD, Universités de Provence et de la Méditerranée, 163 avenue de Luminy, case 925, F-13288 Marseille cedex 9, France.
出版信息
Int J Syst Evol Microbiol. 2011 Jan;61(Pt 1):99-104. doi: 10.1099/ijs.0.017715-0. Epub 2010 Feb 19.
A novel strictly anaerobic, moderately halophilic and mesophilic bacterium, designated strain SOL3f37(T), was isolated from a hydrocarbon-polluted soil surrounding a deep petroleum environment located in south Tunisia. Cells of strain SOL3f37(T) stained Gram-positive and were motile, straight and spore-forming. Strain SOL3f37(T) had a typical Gram-positive-type cell-wall structure, unlike the thick, multilayered cell wall of its closest relative Clostridiisalibacter paucivorans. The major fatty acids were iso-C(15 : 0) (41 %), iso-C(14 : 0) 3-OH and/or iso-C(15 : 0) dimethyl acetal (21.6 %), iso-C(13 : 0) (4.4 %), anteiso-C(15 : 0) (3.9 %) and iso-C(15 : 1) (2.8 %). Strain SOL3f37(T) grew between 20 and 48 °C (optimum 40 °C) and at pH 6.2-8.1 (optimum pH 6.9). Strain SOL3f37(T) required at least 0.5 NaCl l(-1) and grew in the presence of NaCl concentrations up to 150 g l(-1) (optimum 40 g l(-1)). Yeast extract (2 g l(-1)) was required for degradation of pyruvate, fumarate, fructose, glucose and mannitol. Also, strain SOL3f37(T) grew heterotrophically on yeast extract, peptone and bio-Trypticase, but was unable to grow on Casamino acids. Sulfate, thiosulfate, sulfite, elemental sulfur, fumarate, nitrate and nitrite were not reduced. The DNA G+C content was 30.7 mol%. Phylogenetic analysis based on 16S rRNA gene sequences revealed that strain SOL3f37(T) was a member of the family Clostridiaceae in the order Clostridiales; strain SOL3f37(T) was related to members of various genera of the family Clostridiaceae. It exhibited highest 16S rRNA gene sequence similarity (93.4 %) with Clostridiisalibacter paucivorans 37HS60(T), 91.8 % with Thermohalobacter berrensis CTT3(T) and 91.7 % with Caloranaerobacter azorensis MV1087(T). On the basis of genotypic, phenotypic and phylogenetic data, it is suggested that strain SOL3f37(T) represents a novel species in a new genus. The name Sporosalibacterium faouarense gen. nov., sp. nov. is proposed, with SOL3f37(T) (=DSM 21485(T) =JCM 15487(T)) as the type strain of Sporosalibacterium faouarense.
一株新型严格厌氧菌，兼性嗜盐和中温菌，命名为 SOL3f37(T) 菌株，从突尼斯南部一个深石油环境周围污染石油的土壤中分离得到。SOL3f37(T) 菌株的细胞呈革兰氏阳性，运动，直且产芽孢。SOL3f37(T) 菌株具有典型的革兰氏阳性细胞壁结构，与最近的亲缘关系 Clostridiisalibacter paucivorans 的厚多层细胞壁不同。主要脂肪酸为 iso-C(15：0)(41%)、iso-C(14：0)3-OH 和/或 iso-C(15：0)二甲缩醛(21.6%)、iso-C(13：0)(4.4%)、anteiso-C(15：0)(3.9%)和 iso-C(15：1)(2.8%)。SOL3f37(T) 菌株在 20 到 48°C(最佳 40°C)和 pH6.2-8.1(最佳 pH6.9)之间生长。SOL3f37(T) 菌株至少需要 0.5%NaCl(l-1)才能生长，并能在高达 150 g l-1(最佳 40 g l-1)的 NaCl 浓度下生长。丙酮酸、富马酸、果糖、葡萄糖和甘露醇的降解需要酵母提取物(2 g l-1)。此外，SOL3f37(T) 菌株可异养生长在酵母提取物、蛋白胨和生物酪蛋白上，但不能在 Casamino acids 上生长。硫酸盐、硫代硫酸盐、亚硫酸盐、元素硫、富马酸、硝酸盐和亚硝酸盐不能被还原。DNA G+C 含量为 30.7 mol%。基于 16S rRNA 基因序列的系统发育分析表明，SOL3f37(T) 菌株是梭菌科梭菌目梭菌科的成员；SOL3f37(T) 菌株与梭菌科的各种属的成员关系密切。它与 Clostridiisalibacter paucivorans 37HS60(T) 的 16S rRNA 基因序列相似度最高(93.4%)，与 Thermohalobacter berrensis CTT3(T) 的相似度为 91.8%，与 Caloranaerobacter azorensis MV1087(T) 的相似度为 91.7%。基于基因型、表型和系统发育数据，建议 SOL3f37(T) 菌株代表一个新属中的一个新型物种。建议使用 Sporosalibacterium faouarense gen. nov., sp. nov.，其中 SOL3f37(T)=DSM 21485(T)=JCM 15487(T) 作为 Sporosalibacterium faouarense 的模式菌株。
Zotero 插件