Fisher Luke A, Dangre Saloni R, Odenheimer Arthur, Patel Nirav, Doran Peter T, Bowman Jeff S, Schmidt Britney E, Bartlett Douglas H
Scripps Institution of Oceanography, University of California San Diego, La Jolla, CA, USA.
University of California Los Angeles, Los Angeles, CA, USA.
Int J Syst Evol Microbiol. 2025 Apr;75(4). doi: 10.1099/ijsem.0.006760.
A novel, strictly anaerobic, slightly alkaliphilic, halotolerant, peptide- and amino acid-utilizing bacterial strain, SD1, was isolated from a hypersaline lake in Western Australia. The strain stained Gram-negative and was a motile, spore-forming rod. The strain grew between 15 and 50 °C (optimum 40 °C), 1-15% w/v sodium chloride (optimum 5%) and pH 6.0-10.0 (optimum 9.0). Major fatty acids included anteiso-C15 : 0 (24.9%), C14 : 0 dimethyl acetyl (13.2%), anteiso-C15 : 0 dimethyl acetyl (11.5%) and iso-C15 : 0 (10.4%). The DNA G+C content was 30.3 mol%. The isolate did not grow using any tested sugars but grew well on arginine and glycine. It is capable of using elemental sulfur and thiosulfate as alternate electron acceptors, but not sulfide, sulfate, nitrate or nitrite. 16S rRNA gene similarity indicates that the isolate is related to MRo-4 (94.33% identity). SD1 showed 76.18%-76.31% average nucleotide identity with other strains within the family . Phylogenetics, based on the 16S rRNA gene and whole-genome sequence, as well as phenotypic analysis, differentiates the isolate from close neighbors. We propose that SD1 represents a novel species in a new genus, which we have named gen. nov., sp. nov., type strain SD1 (DSM 117405 = TSD-443). From this work, we also propose repositioning of the genus to the family .
从西澳大利亚的一个高盐湖中分离出了一种新型的严格厌氧、微嗜碱、耐盐、利用肽和氨基酸的细菌菌株SD1。该菌株革兰氏染色阴性，是一种能运动、形成芽孢的杆菌。该菌株在15至50°C（最适温度40°C）、1-15%（w/v）氯化钠（最适浓度5%）和pH 6.0-10.0（最适pH 9.0）的条件下生长。主要脂肪酸包括anteiso-C15 : 0（24.9%）、C14 : 0二甲基乙酰（13.2%）、anteiso-C15 : 0二甲基乙酰（11.5%）和iso-C15 : 0（10.4%）。DNA G+C含量为30.3 mol%。该分离株不能利用任何测试的糖类生长，但在精氨酸和甘氨酸上生长良好。它能够利用元素硫和硫代硫酸盐作为替代电子受体，但不能利用硫化物、硫酸盐、硝酸盐或亚硝酸盐。16S rRNA基因相似性表明该分离株与MRo-4相关（同一性为94.33%）。SD1与该科内的其他菌株显示出76.18%-76.31%的平均核苷酸同一性。基于16S rRNA基因和全基因组序列的系统发育分析以及表型分析，将该分离株与近缘菌株区分开来。我们提出SD1代表一个新属中的一个新物种，我们将其命名为gen. nov., sp. nov., 模式菌株SD1（DSM 117405 = TSD-443）。通过这项工作，我们还建议将属重新定位到科。
