Laboratoire de Microbiologie IRD, Aix-Marseille Université, Université du Sud Toulon-Var, CNRS/INSU, IRD, MI0, UM110, 163 avenue de Luminy, case 925, F-13288 Marseille cedex 9, France.
Laboratoire de Microbiologie, Groupe des Bioprocédés, Institut Pasteur de Tunis (IPT), BP 74, 13 place Pasteur, Belvédère-1002, Tunis, Tunisia.
Int J Syst Evol Microbiol. 2012 Oct;62(Pt 10):2469-2474. doi: 10.1099/ijs.0.036566-0. Epub 2011 Dec 2.
A strictly anaerobic, halotolerant and thermotolerant strain, designated C5BEL(T), was isolated in north Tunisia from storage tanks holding waste generated by the recycling of discarded motor oils. Cells of strain C5BEL(T) were Gram-stain-positive, motile by laterally inserted flagella, straight, and spore-forming. Their two major fatty acids were iso-C(15 : 0) and iso-C(15 : 0) dimethyl acetal. Growth was observed at temperatures of 25-55 °C (optimum, 40 °C) and at pH 6-9 (optimum, pH 7.5). The salinity range for growth was 0-100 g l(-1) NaCl (optimum, 5 g l(-1)). Yeast extract was required for growth. Strain C5BEL(T) was heterotrophic, able to use glucose, pyruvate, succinate, yeast extract, bio-trypticase and peptone, but unable to grow on Casamino acids. Sulfate, thiosulfate, sulfite, elemental sulfur, fumarate, nitrate and nitrite were not reduced. The DNA G+C content of strain C5BEL(T) was 31.1 mol%. 16S rRNA gene sequence analysis indicated that strain C5BEL(T) was a member of the family Clostridiaceae, class Clostridia, phylum Firmicutes and was most closely related to Sporanaerobacter acetigenes Lup33(T) ( = DSM 13106(T)) (92.4 % similarity). On the basis of 16S rRNA gene sequence comparisons and physiological characteristics, strain C5BEL(T) can be classified as a novel species in a new genus, for which the name Anaerosalibacter bizertensis gen. nov., sp. nov. is proposed. The type strain of the type species is C5BEL(T) ( = DSM 23801(T) = JCM 17239(T)).
一株严格厌氧、嗜盐和耐热的菌株，被命名为 C5BEL(T)，是从突尼斯北部的储存罐中分离出来的，这些储存罐中存放着回收废弃机油产生的废物。C5BEL(T) 菌株的细胞革兰氏阳性，通过侧向插入的鞭毛运动，呈直杆状，能形成孢子。其两个主要脂肪酸是 iso-C(15 : 0)和 iso-C(15 : 0)二甲缩醛。该菌株在 25-55 °C（最佳温度为 40 °C）和 pH 6-9（最佳 pH 为 7.5）的温度下生长。其生长盐度范围为 0-100 g l(-1) NaCl（最佳盐度为 5 g l(-1)）。该菌株需要酵母提取物才能生长。C5BEL(T) 菌株是异养的，能够利用葡萄糖、丙酮酸盐、琥珀酸盐、酵母提取物、生物胰蛋白酶和蛋白胨，但不能利用 Casamino acids 生长。硫酸盐、硫代硫酸盐、亚硫酸盐、元素硫、富马酸盐、硝酸盐和亚硝酸盐不能被还原。C5BEL(T) 菌株的 DNA G+C 含量为 31.1 mol%。16S rRNA 基因序列分析表明，C5BEL(T) 菌株是梭菌科、梭菌纲、Firmicutes 门的成员，与 Sporanaerobacter acetigenes Lup33(T)（=DSM 13106(T)）（相似度为 92.4%）最为密切相关。基于 16S rRNA 基因序列比较和生理特性，C5BEL(T) 菌株可被分类为一个新属的新种，现提议将其命名为 Bizertensis 属 Anaerosalibacter bizertensis 种 nov.，其模式菌株为 C5BEL(T)（=DSM 23801(T)=JCM 17239(T)）。
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