Department of Physiology, School of Veterinary, Murcia University, Murcia 30100, Spain.
Asian J Androl. 2010 May;12(3):431-41. doi: 10.1038/aja.2009.92. Epub 2010 Feb 22.
In this study, we evaluated the effects of genistein supplementation of the thawing extender on frozen-thawed human semen parameters. We analyzed the effect of supplementation on sperm motility, capacitation (membrane lipid disorder), reactive oxygen species (ROS) generation, chromatin condensation and DNA damage. Using this preliminary information, it maybe possible to improve the cryopreservation process and reduce the cellular damage. We have confirmed that the isoflavone genistein (10 micromol L(-1)) has antioxidant properties on the frozen-thawed spermatozoa. This results in a decreased ROS production that shows a slight improvement in the sperm motility, and decreases the membrane lipid disorder and DNA damage caused by cryopreservation. These results suggest an effect of genistein on sperm functionality that could be of interest for assisted reproduction treatments using frozen-thawed human spermatozoa, but further studies will be necessary to confirm our findings and to evaluate the possible clinical applications.
在这项研究中，我们评估了在解冻液中添加染料木黄酮对冻融人精液参数的影响。我们分析了添加物对精子活力、获能（膜脂紊乱）、活性氧（ROS）生成、染色质浓缩和 DNA 损伤的影响。利用这些初步信息，有可能改善冷冻保存过程并减少细胞损伤。我们已经证实，异黄酮染料木黄酮（10 微摩尔/升）对冻融精子具有抗氧化特性。这导致 ROS 生成减少，显示精子活力略有改善，并减少冷冻保存引起的膜脂紊乱和 DNA 损伤。这些结果表明染料木黄酮对精子功能的影响可能对使用冻融人精子的辅助生殖治疗有意义，但需要进一步研究来证实我们的发现并评估可能的临床应用。
