Jalili Cyrus, Ahmadi Sharareh, Roshankhah Shiva, Salahshoor MohammadReza
Fertility and Infertility Research Center, Kermanshah University of Medical Sciences, Kermanshah, Iran.
Students Research Committee, Kermanshah University of Medical Sciences, Kermanshah, Iran.
Int J Reprod Biomed. 2016 Feb;14(2):95-102.
The predominant phytoestrogen in soy and derived products is the isoflavone Genistein. Genistein has antioxidant properties. Morphine is a main psychoactive chemical in opium that can increase the generation of free radicals and therefore it could adversely affects the spermatogenesis.
The main goal was to investigate whether the Genistein could protect morphine adverse effects on sperm cells viability, count, motility, and testis histology and testosterone hormone and nitric oxide in blood serum.
In this study, various doses of Genistein (0, 1, 2, and 3 mg/kg) and Genistein plus morphine (0, 1, 2, and 3 mg/kg) were administered interaperitoneally to 48 male mice for 30 consequent days. These mice were randomly assigned to 8 groups (n=6) and sperm parameters (sperm cells viability, count, motility and morphology), testis weight and histology, testosterone hormone (ELISA method), FSH and LH hormones (immunoradiometry) and serum nitric oxide (griess assay) were analyzed and compared.
The results indicated that morphine administration significantly decreased testosterone (0.03 ng/mg) LH and FSH level, histological parameters, count, viability (55.3%), morphology and motility of sperm cells (1%), testis weight (0.08 gr) and increase nitric oxide compared to saline group (p=0.00). However, administration of Genistein and Genistein plus morphine significantly boosted motility, morphology, count, viability of sperm cells, seminiferous tubules diameter, germinal thickness, testosterone, LH and FSH while decrease nitric oxide level in all groups compared to morphine group (p<0.025).
It seems that Genistein administration could increase the quality of spermatozoa and prevent morphine- induced adverse effects on sperm parameters.
大豆及其制品中的主要植物雌激素是异黄酮染料木黄酮。染料木黄酮具有抗氧化特性。吗啡是鸦片中的主要精神活性化学物质，可增加自由基的产生，因此可能对精子发生产生不利影响。
主要目的是研究染料木黄酮是否能保护精子细胞活力、数量、运动能力以及睾丸组织学、血清睾酮激素和一氧化氮免受吗啡的不良影响。
在本研究中，将不同剂量的染料木黄酮（0、1、2和3毫克/千克）以及染料木黄酮加吗啡（0、1、2和3毫克/千克）腹腔注射给48只雄性小鼠，连续注射30天。这些小鼠被随机分为8组（每组n = 6），并对精子参数（精子细胞活力、数量、运动能力和形态）、睾丸重量和组织学、睾酮激素（酶联免疫吸附测定法）、促卵泡激素和促黄体生成素激素（免疫放射测定法）以及血清一氧化氮（格里斯测定法）进行分析和比较。
结果表明，与生理盐水组相比，给予吗啡显著降低了睾酮（0.03纳克/毫克）、促黄体生成素和促卵泡激素水平、组织学参数、精子数量、活力（55.3%）、形态和运动能力（1%）、睾丸重量（0.08克），并增加了一氧化氮水平（p = 0.00）。然而，与吗啡组相比，给予染料木黄酮以及染料木黄酮加吗啡显著提高了精子细胞的运动能力、形态、数量、活力、生精小管直径、生精厚度、睾酮、促黄体生成素和促卵泡激素，同时降低了所有组的一氧化氮水平（p < 0.025）。
似乎给予染料木黄酮可以提高精子质量，并预防吗啡对精子参数的不良影响。
