Njung'e K, Handley S L
Pharmaceutical Sciences Institute, Aston University, Birmingham, UK.
Pharmacol Biochem Behav. 1991 Jan;38(1):63-7. doi: 10.1016/0091-3057(91)90590-x.
On individual placement in a cage with 20 evenly spaced glass marbles, female MF1 mice buried 7.8 +/- 0.2 marbles. Olfactory stimuli from experimenters hands and sex of mice had no influence on number buried, but most marbles were buried when they were evenly spaced. There was no habituation to these novel objects on serial testing or prehousing with marbles and, in a two-compartment box, mice did not avoid marbles, spending half their time on the marble side. In the pharmacological experiments, locomotor activity was measured separately to indicate the possibility of nonspecific effects. The anxiogenic agents yohimbine and ethyl-beta-carboline-3-carboxylate (beta-CCE) did not enhance burying, yohimbine decreased burying at doses also reducing locomotor activity. Diazepam effects depended on dose: 0.1 mg/kg increased burying, 0.25 mg/kg had no effect and 1.0-5.0 mg/kg reduced it. Diazepam increased locomotor activity from 0.1-2.5 mg/kg and had no effect at 5.0 mg/kg. Zimeldine, 10.0 mg/kg, reduced burying but not locomotor activity. Inhibition of marble burying may be a correlational model for detection of anxiolytics rather than an isomorphic model of anxiety.
将雌性MF1小鼠单独放置在一个装有20个等距分布玻璃弹珠的笼子里时，它们会掩埋7.8±0.2个弹珠。实验者手部的嗅觉刺激以及小鼠的性别对掩埋弹珠的数量没有影响，但当弹珠均匀分布时，大多数弹珠会被掩埋。在连续测试或预先与弹珠饲养时，小鼠对这些新物体没有产生习惯化，并且在一个两隔间的盒子里，小鼠不会避开弹珠，它们在有弹珠的一侧花费一半的时间。在药理学实验中，分别测量运动活性以表明非特异性效应的可能性。致焦虑剂育亨宾和β-咔啉-3-羧酸乙酯（β-CCE）不会增强掩埋行为，育亨宾在降低运动活性的剂量下会减少掩埋行为。地西泮的作用取决于剂量：0.1mg/kg增加掩埋行为，0.25mg/kg无影响，1.0 - 5.0mg/kg则减少掩埋行为。地西泮在0.1 - 2.5mg/kg时增加运动活性，在5.0mg/kg时无影响。10.0mg/kg的齐美利定减少掩埋行为，但不影响运动活性。抑制弹珠掩埋可能是检测抗焦虑药的一种相关模型，而不是焦虑的同构模型。
