Pharmacology Department, Tsukuba Research Laboratories, GlaxoSmithKline K.K., Tsukuba, Japan.
Pharmacology. 2010;86(5-6):293-6. doi: 10.1159/000321190. Epub 2010 Nov 2.
In the marble burying test, we focused on the 5 distinctive behavioral parameters of mice other than burying marbles, i.e. digging, latency to the first digging, exploration around marbles, rearing and locomotor activity. Typical anxiolytics or antidepressants with different mechanisms, fluvoxamine (30 mg/kg, selective serotonin reuptake inhibitor), bupropion (60 mg/kg, noradrenaline and dopamine reuptake inhibitor), imipramine (60 mg/kg, tricyclic antidepressant) and diazepam (10 mg/kg, benzodiazepine) were used to examine whether these behavioral parameters are sensitive to pharmacological treatments. Each of the drugs demonstrated an individual action pattern on the 4 behavioral parameters (latency to the first digging, exploration around marbles, rearing and locomotor activity). On the other hand, all 4 drugs reduced burying marbles and digging, which were correlated with each other. These results suggest that the former 4 behavioral parameters are sensitive to pharmacological treatment and that pharmacological regulation mechanisms of them may be different from burying marbles and digging. They could be useful to identify the type of action of a test drug like selective serotonin reuptake inhibitor, noradrenaline and dopamine reuptake inhibitor, tricyclic antidepressant or benzodiazepine.
在大理石掩埋测试中，我们关注的是老鼠除了掩埋大理石之外的 5 种不同的行为参数，即挖掘、第一次挖掘的潜伏期、围绕大理石的探索、后肢站立和运动活动。不同机制的典型抗焦虑药或抗抑郁药，如氟伏沙明（30mg/kg，选择性 5-羟色胺再摄取抑制剂）、安非他酮（60mg/kg，去甲肾上腺素和多巴胺再摄取抑制剂）、丙咪嗪（60mg/kg，三环抗抑郁药）和地西泮（10mg/kg，苯二氮䓬类），被用于检查这些行为参数是否对药物治疗敏感。每种药物在 4 种行为参数（第一次挖掘的潜伏期、围绕大理石的探索、后肢站立和运动活动）上表现出不同的作用模式。另一方面，所有 4 种药物均减少了大理石掩埋和挖掘，这两种行为参数之间存在相关性。这些结果表明，前 4 种行为参数对药物治疗敏感，其药物调节机制可能与大理石掩埋和挖掘不同。这些行为参数可能有助于确定测试药物（如选择性 5-羟色胺再摄取抑制剂、去甲肾上腺素和多巴胺再摄取抑制剂、三环抗抑郁药或苯二氮䓬类）的作用类型。
