State Key Laboratory of Coordination Chemistry, School of Chemistry and Chemical Engineering, Nanjing University, Nanjing, 210093, P. R. China.
Analyst. 2010 Mar;135(3):559-63. doi: 10.1039/b920985a. Epub 2010 Jan 8.
Bacterial magnetic nanoparticles (BMP) are used in rapid enrichment and determination of phosphorylated peptides. Fe(3+) and Zr(4+) can be easily immobilized on the surface of BMP without any other modification, and the metal ion immobilized BMP can enrich phosphopeptides rapidly through strong coordination bonding. It is found that the Zr(4+)-immobilized BMP can enrich the multiphosphorylated peptides while Fe(3+)-immobilized BMP preferred the singly phosphorylated peptides. Fe(3+)-immobilized BMP could detect phosphorylated peptides under lower concentration than that of Zr(4+) owing to the larger amount of Fe(3+) on BMPs. Besides, BMP itself can enrich one kind of phosphorylated peptide from alpha-casein digest directly through weak interactions between BMP and phosphorylated peptides.
细菌磁纳米颗粒（BMP）用于快速富集和测定磷酸化肽。Fe(3+)和 Zr(4+)可以很容易地固定在 BMP 的表面上，而无需任何其他修饰，并且金属离子固定的 BMP 可以通过强配位键快速富集磷酸肽。研究发现，Zr(4+)-固定的 BMP 可以富集多磷酸化肽，而 Fe(3+)-固定的 BMP 则优先富集单磷酸化肽。由于 BMP 上的 Fe(3+)较多，因此 Fe(3+)-固定的 BMP 可以在较低浓度下检测到磷酸化肽。此外，BMP 本身可以通过 BMP 与磷酸化肽之间的弱相互作用，直接从α-酪蛋白消化物中富集一种磷酸化肽。
