Lee Aera, Yang Hyo-Jik, Lim Eun-Soo, Kim Jeongkwon, Kim Yangsun
Department of Chemistry, Chungnam National University, Daejeon 305-764, South Korea.
Rapid Commun Mass Spectrom. 2008 Aug;22(16):2561-4. doi: 10.1002/rcm.3652.
Magnetic iron(II, III) oxide (magnetite, Fe(3)O(4)) nanoparticles were used to selectively enrich phosphopeptides from tryptic digests of bovine beta-casein and from tryptic digest mixtures containing bovine beta-casein, cytochrome c, bovine serum albumin, and horse heart myoglobin. The magnetic property of the particles permits an easy and speedy enrichment process. No enrichment of phosphopeptides was observed from ferric magnetic iron(III) oxide (Fe(2)O(3)) nanoparticles. These data collectively demonstrate that the enrichment of phosphopeptides using magnetic iron(II, III) oxide nanoparticles is a practical method for the selective analysis of phosphopeptides and could be helpful in isolating and analyzing phosphorylated peptides from complex biological samples.
磁性铁（II，III）氧化物（磁铁矿，Fe₃O₄）纳米颗粒用于从牛β-酪蛋白的胰蛋白酶消化物以及含有牛β-酪蛋白、细胞色素c、牛血清白蛋白和马心肌红蛋白的胰蛋白酶消化混合物中选择性富集磷酸肽。颗粒的磁性使富集过程简便快捷。从三氧化二铁（Fe₂O₃）磁性纳米颗粒中未观察到磷酸肽的富集。这些数据共同表明，使用磁性铁（II，III）氧化物纳米颗粒富集磷酸肽是一种用于磷酸肽选择性分析的实用方法，有助于从复杂生物样品中分离和分析磷酸化肽。
