State Key Laboratory for Chemo/Biosensing and Chemometrics, College of Chemistry and Chemical Engineering, Hunan University, Changsha 410082, P. R. China.
Analyst. 2010 Mar;135(3):570-6. doi: 10.1039/b922382g. Epub 2010 Jan 15.
Here we describe the biocatalytic growth of high-density gold agglomerates on a gold electrode surface to form a carrier for aptamer probe immobilization. The present approach provides a simple strategy to promote the seed-mediated deposition of Au from AuCl(4)(-) onto surface-attached 12 nm diameter Au nanoparticles (AuNPs) in the presence of reductive coenzyme and surfactant. The growth process was studied by electrochemical impedance spectroscopy (EIS) and scanning electron microscopy (SEM). This nanostructured platform is effective and prospective toward the aptamer probe immobilization. For the nice performance of enhanced substrate, the aptamer-sensing interface showed excellent applicability under the investigations such as alternating current voltammetry (ACV) and surface-enhanced Resonance Raman scattering (SERRS) spectra.
在这里，我们描述了在金电极表面上通过生物催化生长高密度的金团聚体，以形成适体探针固定化的载体。本方法提供了一种简单的策略，即在还原辅酶和表面活性剂的存在下，促进从 AuCl(4)(-)到表面附着的 12nm 直径金纳米粒子（AuNPs）上的 Au 的种子介导沉积。通过电化学阻抗谱（EIS）和扫描电子显微镜（SEM）研究了生长过程。这种纳米结构平台对于适体探针的固定化是有效和有前景的。对于增强基底的良好性能，适体传感界面在交流伏安法（ACV）和表面增强共振拉曼散射（SERRS）光谱等研究中表现出优异的适用性。
