School of Chemistry and Environment, Beijing University of Aeronautics and Astronautics, Haidian District, Beijing, China.
Talanta. 2011 Feb 15;83(5):1501-6. doi: 10.1016/j.talanta.2010.11.042. Epub 2010 Nov 26.
A simple, highly sensitive, and label-free electrochemical impedance spectroscopy (EIS) aptasensor based on an anti-lysozyme-aptamer as a molecular recognition element, was developed for the detection of lysozyme. Improvement in sensitivity was achieved by utilizing gold nanoparticles (AuNPs), which were electrodeposited onto the surface of a gold electrode, as a platform for immobilization of the aptamer. To quantify the amount of lysozyme, changes in the interfacial electron transfer resistance (R(et)) of the aptasensor were monitored using the redox couple of an Fe(CN)(6) probe. The R(et) increased with lysozyme concentration. The plot of R(et) against the logarithm of lysozyme concentration is linear over the range from 0.1 pM to 500 pM with a detection limit of 0.01 pM. The aptasensor also showed good selectivity for lysozyme without being affected by the presence of other proteins.
基于抗溶菌酶适体作为分子识别元件的简单、高灵敏、无需标记的电化学阻抗谱（EIS）适体传感器被开发出来，用于检测溶菌酶。通过利用金纳米粒子（AuNPs）作为适体固定的平台，提高了灵敏度。为了定量溶菌酶的含量，使用 Fe(CN)(6) 探针的氧化还原对监测适体传感器的界面电子转移电阻（R(et)）的变化。随着溶菌酶浓度的增加，R(et) 增加。R(et) 与溶菌酶浓度的对数之间的关系在 0.1 pM 至 500 pM 的范围内呈线性关系，检测限为 0.01 pM。该适体传感器对溶菌酶具有良好的选择性，不受其他蛋白质的影响。
