Department of Pharrmaceutical Biology, Institute of Pharmacy, Free University of Berlin, Königin-Luise-Str. 2+4, 14195, Berlin, Germany.
In Vitro Cell Dev Biol Anim. 2010 Jun;46(6):492-6. doi: 10.1007/s11626-010-9273-7. Epub 2010 Feb 21.
The main purpose of this study was to investigate lysozyme synthesis and secretion in three human monocyte cell lines: U-937, HL-60, and THP-1, using sensitive fluorescence-based assay of lysozyme activity. PMA and hIFN-gamma were evaluated for inducing lysozyme activity. Using well-defined cell lines from the cell culture collection, no lysozyme activity could be detected in the cultured U-937 cells either with or without addition of the inducing factors. These data suggested, contrary to previous reports, that U-937 cell line cannot synthesize or secrete active lysozyme. THP-1 and HL-60 cells were proved to produce enzymatically active lysozyme in increasing amounts with the time course. PMA and hIFN-gamma had no significant inducing effect on the production or the release of active lysozyme in THP-1 and HL-60 cells. We showed inhibiting effect of PMA and hIFN-gamma on the lysozyme activity, particularly in HL-60 cell line.
本研究的主要目的是使用溶菌酶活性的灵敏荧光测定法，研究三种人单核细胞系（U-937、HL-60 和 THP-1）中溶菌酶的合成和分泌。评估 PMA 和 hIFN-γ诱导溶菌酶活性的能力。使用细胞培养库中明确界定的细胞系，无论是否添加诱导因子，在培养的 U-937 细胞中均无法检测到溶菌酶活性。这些数据表明，与之前的报告相反，U-937 细胞系不能合成或分泌具有活性的溶菌酶。THP-1 和 HL-60 细胞被证明可以随着时间的推移以增加的量产生具有酶活性的溶菌酶。PMA 和 hIFN-γ对 THP-1 和 HL-60 细胞中活性溶菌酶的产生或释放没有显著的诱导作用。我们显示了 PMA 和 hIFN-γ对溶菌酶活性的抑制作用，特别是在 HL-60 细胞系中。
