Uppsala BioCenter, Department of Forest Mycology and Pathology, Swedish University of Agricultural Sciences, P.O. Box 7026, 75007 Uppsala, Sweden.
Mycorrhiza. 2010 Oct;20(7):505-9. doi: 10.1007/s00572-010-0299-1. Epub 2010 Feb 20.
The objectives of this study were to investigate impact of stump and slash removal on growth and mycorrhization of Picea abies seedlings outplanted on a forest clear-cut. Four non-replicated site preparation treatments included: (1) mounding (M), (2) removal of stumps (K), (3) mounding and removal of logging slash (HM) and (4) removal of logging slash and stumps (HK). Results showed that height increment of the seedlings was highest in K and lowest in M after the third growing season, and similar pattern remained after the fourth season. Ectomycorrhizal (ECM) colonisation of seedling roots was highest in M (96.6%) and lowest in K (72.3%), and even in HK (76.0%) and HM (76.3%). Morphotyping and sequencing of internal transcribed spacer of fungal ribosomal DNA revealed a total of 13 ECM species. Among those, Thelephora terrestris and Cenococcum geophilum were the most common, found on 27.4% and 26.3% of roots, respectively. The rest of species colonised 26.6% of roots. Richness of ECM species was highest in M (10 species) and lowest in K (three species). Consequently, stump and slash removal from clear-felled sites had a positive effect on growth of outplanted spruce seedlings, but negative effect on their mycorrhization. This suggests that altered soil conditions due to site disturbance by stump and slash removal might be more favourable for tree growth than more abundant mycorrhization of their root systems in less disturbed soil.
本研究的目的是调查伐桩和伐根清除对造林后森林皆伐迹地上云杉幼苗生长和菌根形成的影响。四个非重复的整地处理包括：（1）堆土（M），（2）清除伐桩（K），（3）堆土和清除采伐剩余物（HM），以及（4）清除采伐剩余物和伐桩（HK）。结果表明，在第三个生长季后，幼苗的高度增量在 K 最高，在 M 最低，第四个生长季后仍保持相同模式。在 M 中，幼苗根系的外生菌根（ECM）定殖率最高（96.6%），在 K 中最低（72.3%），甚至在 HK（76.0%）和 HM（76.3%）中也是如此。真菌核糖体 DNA 内转录间隔区的形态和测序显示，共有 13 种 ECM 物种。其中，地衣状革耳菌和共生菌是最常见的，分别在 27.4%和 26.3%的根上发现。其余物种定殖了 26.6%的根。ECM 物种的丰富度在 M 中最高（10 种），在 K 中最低（3 种）。因此，从皆伐迹地清除伐桩和伐根对造林云杉幼苗的生长有积极影响，但对其菌根形成有消极影响。这表明，由于清除伐桩和伐根导致的迹地土壤条件的改变，可能比在干扰较小的土壤中更丰富的根系菌根化对树木生长更有利。
