Lynikienė Jūratė, Marčiulynienė Diana, Marčiulynas Adas, Gedminas Artūras, Vaičiukynė Miglė, Menkis Audrius
Institute of Forestry, Lithuanian Research Centre for Agriculture and Forestry, Liepų str. 1, Girionys, LT-53101 Kaunas, Lithuania.
Department of Forest Mycology and Plant Pathology, Uppsala BioCenter, Swedish University of Agricultural Sciences, P.O. Box 7026, SE-75007 Uppsala, Sweden.
Microorganisms. 2020 Feb 15;8(2):259. doi: 10.3390/microorganisms8020259.
The aim was to assess fungal communities associated with living needles and soil of in managed and unmanaged forest stands to get a better understanding of whether and how different intensities of forest management affects fungal diversity and community composition under the north temperate forest zone conditions. The study was carried out in three national parks in Lithuania. Each included five study sites in managed stands and five in unmanaged stands. At each site, three random soil cores and five random last-year needle samples were collected. Following DNA isolation, a DNA fragment of the ITS2 rRNA gene region of each sample was individually amplified and subjected to high-throughput sequencing. Analysis of 195,808 high-quality reads showed the presence of 1909 fungal taxa. Richness and composition of fungal taxa were similar in each substrate (needles and soil) in managed . unmanaged sites. The most common fungi in needles were (12.4% of all fungal sequences), Unidentified sp. 3980_1 (12.4%), Unidentified sp. 3980_4 (4.1%) and (3.1%). In soil: Unidentified sp. 3980_21 (8.6%), (8.2%), sp. 3980_5 (8.1%) and (6.3%). The results demonstrated that managed and unmanaged stands support similar diversity and composition of fungal communities associated with living needles and soil.
目的是评估与经营和未经营林分中活针叶和土壤相关的真菌群落，以便更好地了解在北温带森林带条件下，不同强度的森林经营是否以及如何影响真菌多样性和群落组成。该研究在立陶宛的三个国家公园进行。每个公园包括五个经营林分的研究地点和五个未经营林分的研究地点。在每个地点，随机采集三个土壤样本和五个去年的针叶样本。DNA提取后，对每个样本的ITS2 rRNA基因区域的DNA片段进行单独扩增，并进行高通量测序。对195,808条高质量读数的分析显示存在1909个真菌分类单元。在经营和未经营地点，每个基质（针叶和土壤）中真菌分类单元的丰富度和组成相似。针叶中最常见的真菌是（占所有真菌序列的12.4%）、未鉴定的物种3980_1（12.4%）、未鉴定的物种3980_4（4.1%）和（3.1%）。在土壤中：未鉴定的物种3980_21（8.6%）、（8.2%）、物种3980_5（8.1%）和（6.3%）。结果表明，经营和未经营的林分支持与活针叶和土壤相关的真菌群落的相似多样性和组成。
