Department of Renewable Resources, University of Alberta, Edmonton, Canada.
Mycorrhiza. 2014 Aug;24(6):431-41. doi: 10.1007/s00572-014-0555-x. Epub 2014 Jan 15.
The effectiveness of ectomycorrhizal inoculation at the tree nursery seedling production stage on growth and survival was examined in jack pine (Pinus banksiana) and white spruce (Picea glauca) planted in oil sands reclamation sites. The seedlings were inoculated with Hebeloma crustuliniforme strain # UAMH 5247, Suillus tomentosus strain # UAMH 6252, and Laccaria bicolor strain # UAMH 8232, as individual pure cultures and in combinations. These treatments were demonstrated to improve salinity resistance and water uptake in conifer seedlings. The field responses of seedlings to ectomycorrhizal inoculation varied between plant species, inoculation treatments, and measured parameters. Seedling inoculation resulted in higher ectomycorrhizal colonization rates compared with non-inoculated control, which had also a relatively small proportion of roots colonized by the nursery contaminant fungi identified as Amphinema byssoides and Thelephora americana. Seedling inoculation had overall a greater effect on relative height growth rates, dry biomass, and stem volumes in jack pine compared with white spruce. However, when examined after two growing seasons, inoculated white spruce seedlings showed up to 75% higher survival rates than non-inoculated controls. The persistence of inoculated fungi in roots of planted seedlings was examined at the end of the second growing season. Although the inoculation with H. crustuliniforme triggered growth responses, the fungus was not found in the roots of seedlings at the end of the second growing season suggesting a possibility that the observed growth-promoting effect of H. crustuliniforme may be transient. The results suggest that the inoculation of conifer seedlings with ectomycorrhizal fungi could potentially be carried out on a large scale in tree nurseries to benefit postplanting performance in oil sands reclamation sites. However, these practices should take into consideration the differences in responses between the different plant species and fungal strains.
在油砂复垦区种植的湿地松（Pinus banksiana）和白云杉（Picea glauca）的苗圃幼苗生产阶段，研究了外生菌根接种对生长和成活率的影响。将幼苗接种到 Heloboma crustuliniforme 菌株 # UAMH 5247、Suillus tomentosus 菌株 # UAMH 6252 和 Laccaria bicolor 菌株 # UAMH 8232 中，作为单一纯培养物和组合进行接种。这些处理被证明可以提高针叶树幼苗的耐盐性和吸水能力。幼苗对外生菌根接种的田间反应因植物物种、接种处理和测量参数而异。与未接种对照相比，接种后的幼苗外生菌根定殖率更高，而未接种对照的根系中也有相对较小比例的根被鉴定为 Amphinema byssoides 和 Thelephora americana 的苗圃污染真菌定殖。与白云杉相比，接种对湿地松的相对高度生长率、干生物量和茎体积的影响总体更大。然而，在经过两个生长季后检查时，接种的白云杉幼苗的存活率比未接种对照高出高达 75%。在第二个生长季结束时，检查了接种真菌在种植幼苗根系中的持久性。尽管接种 H. crustuliniforme 触发了生长反应，但在第二个生长季结束时，在幼苗的根部未发现该真菌，这表明观察到的 H. crustuliniforme 促进生长的效果可能是暂时的。结果表明，在外生菌根真菌接种针叶树幼苗可以在苗圃中大规模进行，以有益于油砂复垦区的种植后性能。然而，这些做法应考虑到不同植物物种和真菌菌株之间的反应差异。
