采用正交试验设计优化柴胡中柴胡皂苷的超临界流体萃取。

Optimization of supercritical fluid extraction of saikosaponins from Bupleurum falcatum with orthogonal array design.

机构信息

State Key Laboratory of Crop Biology, College of Agronomy, Shandong Agricultural University, Tai'an, PR China.

出版信息

J Sep Sci. 2010 Apr;33(8):1161-6. doi: 10.1002/jssc.200900529.

DOI:10.1002/jssc.200900529

PMID:20175084

Abstract

Supercritical fluid extraction (SFE) was used to extract saikosaponins a, c and d from the root of Bupleurum falcatum. An orthogonal array design L(9)(3)(4) was employed as a chemometric method for the optimization of the SFE conditions. The effects of four factors including pressure (30-40 MPa), temperature (40-50 degrees C), ethanol concentration (60-100%) and time (2.5-3.5 h) on the yields of saikosaponins were investigated by a preparative SFE system in the SFE mode. Under the optimized conditions, namely 35 MPa of pressure, 45 degrees C of temperature, 80% of ethanol concentration and 3.0 h of time, the yields of saikosaponin c, saikosaponin a, saikosaponin d, total saikosaponins and SFE extract were 0.16, 0.12, 0.96, 1.24 and 16.48 mg/g, respectively. Determinations of the saikosaponins were performed by HPLC.

摘要

超临界流体萃取（SFE）用于从柴胡根中提取柴胡皂苷 a、c 和 d。采用正交数组设计 L(9)(3)(4) 作为化学计量学方法，对 SFE 条件进行优化。通过制备型 SFE 系统在 SFE 模式下，考察了压力（30-40 MPa）、温度（40-50°C）、乙醇浓度（60-100%）和时间（2.5-3.5 h）这四个因素对柴胡皂苷收率的影响。在优化条件下，即压力 35 MPa、温度 45°C、乙醇浓度 80%和时间 3.0 h，柴胡皂苷 c、柴胡皂苷 a、柴胡皂苷 d、总柴胡皂苷和 SFE 提取物的收率分别为 0.16、0.12、0.96、1.24 和 16.48 mg/g。采用 HPLC 对柴胡皂苷进行了测定。