State Key Laboratory of Crop Biology, College of Agronomy, Shandong Agricultural University, Tai'an, PR China.
J Sep Sci. 2010 Apr;33(8):1161-6. doi: 10.1002/jssc.200900529.
Supercritical fluid extraction (SFE) was used to extract saikosaponins a, c and d from the root of Bupleurum falcatum. An orthogonal array design L(9)(3)(4) was employed as a chemometric method for the optimization of the SFE conditions. The effects of four factors including pressure (30-40 MPa), temperature (40-50 degrees C), ethanol concentration (60-100%) and time (2.5-3.5 h) on the yields of saikosaponins were investigated by a preparative SFE system in the SFE mode. Under the optimized conditions, namely 35 MPa of pressure, 45 degrees C of temperature, 80% of ethanol concentration and 3.0 h of time, the yields of saikosaponin c, saikosaponin a, saikosaponin d, total saikosaponins and SFE extract were 0.16, 0.12, 0.96, 1.24 and 16.48 mg/g, respectively. Determinations of the saikosaponins were performed by HPLC.
超临界流体萃取（SFE）用于从柴胡根中提取柴胡皂苷 a、c 和 d。采用正交数组设计 L(9)(3)(4) 作为化学计量学方法，对 SFE 条件进行优化。通过制备型 SFE 系统在 SFE 模式下，考察了压力（30-40 MPa）、温度（40-50°C）、乙醇浓度（60-100%）和时间（2.5-3.5 h）这四个因素对柴胡皂苷收率的影响。在优化条件下，即压力 35 MPa、温度 45°C、乙醇浓度 80%和时间 3.0 h，柴胡皂苷 c、柴胡皂苷 a、柴胡皂苷 d、总柴胡皂苷和 SFE 提取物的收率分别为 0.16、0.12、0.96、1.24 和 16.48 mg/g。采用 HPLC 对柴胡皂苷进行了测定。
