Chen Yan, Zhang Cuiping, Zhang Mei, Fu Xiaobing
Department of Molecular Cell Biology, Institute of Basic Medical Science, Chinese PLA General Hospital, Haidian District, Beijing 100853, China ; Department of Pharmacy, General Hospital of Beijing Military Region, Dongcheng District, Beijing 100700, China.
Department of Wound Repair and Tissue Regeneration, The First Affiliated Hospital (304th Hospital), General Hospital of PLA, Beijing 100048, PR China.
Pharmacogn Mag. 2015 Jul-Sep;11(43):435-43. doi: 10.4103/0973-1296.160444.
Since antiquity, Zingiber officinale (ginger), pogostemonis herba, and radix aucklandiae have been used as traditional Chinese medicines to remit gastrointestinal discomfort. Recent evidences also show the efficacy of the three herbal medicines against nausea and vomiting.
To optimize the CO2 supercritical fluid extraction (SFE-CO2) conditions for ginger and the ethanol reflux extraction conditions for radix aucklandiae, control the quality of pogostemonis herba essential oil, and evaluate anti-motion sickness activity of the compound recipes composed of the three herbal medicine extracts.
Two orthogonal array designs L9 (3)(4) were employed to optimize the SFE-CO2 conditions for enhancing yield of 6-gingerol from ginger and the ethanol reflux extraction conditions for enhancing yield of costunolide and dehydrocostus lactone from radix aucklandiae; a uniform design U5(5(3)) was applied for evaluation of anti-motion sickness activity of the compound recipes.
Extraction pressure (P < 0.01), extraction temperature and extraction time (P < 0.05) have significant effects on the yield of 6-gingerol from ginger by SFE-CO2; ethanol concentration (P < 0.01) and times of repeating extraction (P < 0.05) have significant effects on the total yield of costunolide and dehydrocostus lactone from radix aucklandiae by ethanol reflux extraction; the anti-motion sickness effects of the optimized compound recipe composed of the three herbal medicine extracts were markedly better than those of dimenhydrinate.
The compound recipe composed of ginger, pogostemonis herba, and radix aucklandiae could be developed as a promising anti-motion sickness medicine.
自古以来，生姜、广藿香和木香就被用作缓解胃肠道不适的传统中药。近期证据也表明这三种草药对恶心和呕吐有疗效。
优化生姜的二氧化碳超临界流体萃取（SFE-CO₂）条件及木香的乙醇回流提取条件，控制广藿香精油质量，并评价由这三种草药提取物组成的复方的抗晕动病活性。
采用两个L9(3)(4)正交试验设计分别优化生姜中6-姜酚的超临界流体萃取条件以及木香中木香烃内酯和去氢木香内酯的乙醇回流提取条件；采用U5(5(3))均匀设计评价复方的抗晕动病活性。
萃取压力（P<0.01）、萃取温度和萃取时间（P<0.05）对生姜超临界流体萃取6-姜酚的得率有显著影响；乙醇浓度（P<0.01）和提取次数（P<0.05）对木香乙醇回流提取木香烃内酯和去氢木香内酯的总得率有显著影响；由三种草药提取物组成的优化复方的抗晕动病作用明显优于茶苯海明。
生姜、广藿香和木香组成的复方有望开发成为一种抗晕动病药物。
