Carbohydrate and Protein Biotechnology Laboratory, Department of Biotechnology, Punjabi University, Patiala, Punjab, India.
J Basic Microbiol. 2010 Feb;50(1):90-7. doi: 10.1002/jobm.200900299.
Fifteen species of Aspergillus were screened for occurrence of lectins. Nine of them (A. sydowii, A. candidus, A. allahabadi, A. terricola, A. ficuum, A. sparsus, A. carneus, A. pulvinus and A. aculeatus) were found to possess lectin activity. None of the species elaborated lectin in culture supernatant. All the lectins agglutinated rat, pig and rabbit erythrocytes. A. sydowii, A. candidus, A. allahabadi, A. terricola, A. ficuum, A. sparsus, A. carneus and A. aculeatus lectins agglutinated all human type erythrocytes equally, while A. pulvinus lectin specifically agglutinated human type A and O erythrocytes. Neuraminidase and protease treatment to erythrocytes substantially augmented lectin titres manyfold. Lectins showed specificity to mucin and asialofetuin and all of them were specific to L-arabinose except that of A. carneus. Lectins from A. sydowii, A. ficuum, A. sparsus and A. carneus displayed remarkable specificities to D-xylose. Maximum lectin activity was expressed by 11 day old cultures of A. sydowii (titre 32), A. ficuum (titre 64) and A. sparsus (titre 1024). Lectins from A. aculeatus, A. candidus and A. terricola were expressed by 7-10 days, 6-9 days and 5-11 days old cultures, respectively. A. allahabadi cultures exhibited maximum lectin activity (titre 32) after 8-10 days of cultivation. A. carneus and A. pulvinus expressed optimal titres of 32 and 8, respectively on the 9th day.
十五种曲霉被筛选是否存在凝集素。其中九种（棘孢曲霉、白曲霉、长枝木霉、土曲霉、棒曲霉、少孢曲霉、肉色曲霉、突脐孢曲霉和疣孢曲霉）被发现具有凝集素活性。没有一种曲霉在培养上清液中产生凝集素。所有的凝集素都能凝集大鼠、猪和兔的红细胞。棘孢曲霉、白曲霉、长枝木霉、土曲霉、棒曲霉、少孢曲霉、肉色曲霉和疣孢曲霉的凝集素平等地凝集所有人类血型的红细胞，而突脐孢曲霉的凝集素则特异性地凝集人类 A 型和 O 型红细胞。用神经氨酸酶和蛋白酶处理红细胞可使凝集素效价增加数倍。凝集素对粘蛋白和唾液酸胎球蛋白具有特异性，除肉色曲霉外，它们都特异性地识别 L-阿拉伯糖。棘孢曲霉、棒曲霉、少孢曲霉和肉色曲霉的凝集素对 D-木糖表现出显著的特异性。棘孢曲霉（效价 32）、棒曲霉（效价 64）和少孢曲霉（效价 1024）的 11 天龄培养物表达出最大的凝集素活性。棘孢曲霉、白曲霉和土曲霉的凝集素分别在 7-10 天、6-9 天和 5-11 天的培养物中表达，而长枝木霉的凝集素则在 8-10 天的培养物中表达。土曲霉在培养 8-10 天后表现出最大的凝集素活性（效价 32）。肉色曲霉和突脐孢曲霉在第 9 天分别表达出最佳的 32 倍和 8 倍效价。
