Carbohydrate and Protein Biotechnology Laboratory, Department of Biotechnology, Punjabi University, Patiala, Punjab, India.
J Basic Microbiol. 2009 Oct;49(5):471-6. doi: 10.1002/jobm.200800282.
Out of 15 Penicillium species screened for lectin activities, P. griseofulvum and P. thomii were found to possess mycelial lectin activity. None of the species displayed extracellular or cell surface-bound lectin activity. Both species agglutinated rabbit erythrocytes. P. griseofulvum lectin showed specificity to human type O erythrocytes. While P. thomii lectin specifically agglutinated human type A erythrocytes. Highest lectin activities from P. thomii and P. griseofulvum were expressed after 8 and 7 days of growth, respectively. Lectins from both the species displayed a high binding affinity to chondroitin-6-sulphate, mucin, asialofetuin, D-sucrose, and D-trehalose. Ammonium sulphate at 50% saturation yielded 80% of the total lectin activity. Dialysis and ultrafiltration of the precipitates resulted in 1.79 and 3.46 fold purification of P. griseofulvum and P. thomii lectins, respectively. Both lectins showed pH optima between 7.0-8.0 and were stable near the neutral pH after 2 h. P. thomii lectin exhibited optimal activity at 35-40 degrees C, and P. griseofulvum lectin at 30-40 degrees C. P. thomii lectin showed a complete loss of activity above 40 degrees C, P. griseofulvum lectin was stable at or below 35 degrees C.
在筛选出具有凝集素活性的 15 种青霉属物种中，发现灰绿青霉和 Thomii 青霉具有菌丝体凝集素活性。没有一种物种表现出细胞外或细胞表面结合的凝集素活性。两种物种都能凝集兔红细胞。灰绿青霉凝集素对人 O 型红细胞具有特异性。而 Thomii 凝集素则特异性地凝集人 A 型红细胞。Thomii 和灰绿青霉的凝集素活性最高分别在生长 8 天和 7 天后表达。两种物种的凝集素对硫酸软骨素 6-硫酸盐、粘蛋白、去唾液酸胎球蛋白、D-蔗糖和 D-海藻糖具有高结合亲和力。50%饱和度的硫酸铵产生 80%的总凝集素活性。沉淀的透析和超滤分别使灰绿青霉和 Thomii 凝集素的纯化倍数达到 1.79 倍和 3.46 倍。两种凝集素的 pH 最佳范围均为 7.0-8.0，在中性 pH 附近 2 小时后仍保持稳定。Thomii 凝集素在 35-40 摄氏度时表现出最佳活性，而灰绿青霉凝集素在 30-40 摄氏度时表现出最佳活性。Thomii 凝集素的活性在 40 摄氏度以上完全丧失，而灰绿青霉凝集素在 35 摄氏度或以下稳定。
