Departments of Pathology, Microbiology and Immunology/Oncology Institute, Loyola University Chicago, IL, USA.
Pigment Cell Melanoma Res. 2010 Apr;23(2):276-86. doi: 10.1111/j.1755-148X.2010.00688.x. Epub 2010 Feb 19.
In human vitiligo, cutaneous depigmentation involves cytotoxic activity of autoreactive T cells. It was hypothesized that depigmentation can progress in the absence of regulatory T cells (Treg). The percentage of Treg among skin infiltrating T cells was evaluated by immunoenzymatic double staining for CD3 and FoxP3, revealing drastically reduced numbers of Treg in non-lesional, perilesional and lesional vitiligo skin. Assessment of the circulating Treg pool by FACS analysis of CD4, CD25, CD127 and FoxP3 expression, and mixed lymphocyte reactions in presence and absence of sorted Treg revealed no systemic drop in the abundance or activity of Treg in vitiligo patients. Expression of skin homing receptors CCR4, CCR5, CCR8 and CLA was comparable among circulating vitiligo and control Treg. Treg from either source were equally capable of migrating towards CCR4 ligand and skin homing chemokine CCL22, yet significantly reduced expression of CCL22 in vitiligo skin observed by immunohistochemistry may explain failure of circulating, functional Treg to home to the skin in vitiligo. The paucity of Treg in vitiligo skin is likely crucial for perpetual anti-melanocyte reactivity in progressive disease.
在人类白癜风中，皮肤色素脱失涉及自身反应性 T 细胞的细胞毒性活性。据推测，在没有调节性 T 细胞（Treg）的情况下，色素脱失也可能进展。通过免疫酶双重染色 CD3 和 FoxP3 评估皮肤浸润性 T 细胞中的 Treg 百分比，发现非皮损、皮损周围和皮损白癜风皮肤中的 Treg 数量明显减少。通过流式细胞术分析 CD4、CD25、CD127 和 FoxP3 的表达以及存在和不存在分选的 Treg 的混合淋巴细胞反应来评估循环 Treg 池，并未发现白癜风患者中 Treg 的丰度或活性出现全身性下降。循环白癜风和对照 Treg 之间的皮肤归巢受体 CCR4、CCR5、CCR8 和 CLA 的表达无差异。来自任何来源的 Treg 都能够同样地向 CCR4 配体和皮肤归巢趋化因子 CCL22 迁移，但免疫组化观察到白癜风皮肤中 CCL22 的表达明显减少，可能解释了循环、功能性 Treg 未能在白癜风中归巢到皮肤的原因。白癜风皮肤中 Treg 的缺乏可能对进行性疾病中持续的抗黑素细胞反应至关重要。
