Galloway M
Royal Free Hampstead NHS Trust/UCL Medical School, Department of Cellular Pathology, Royal Free Hospital, Pond Street, London, UK.
Clin Neuropathol. 2010 Mar-Apr;29(2):89-93. doi: 10.5414/npp29089.
This study aimed to determine whether CD34 is expressed in glioblastomas and giant cell glioblastomas, as this information may be of value when attempting to differentiate between giant cell glioblastomas and other relevant differential diagnoses such as pleomorphic xanthoastrocytomas with anaplastic features and anaplastic gangliogliomas.
11 giant cell glioblastomas and 16 non-giant cell glioblastomas were assessed with immunocytochemical staining for CD34.
Standard immunocytochemical techniques were used, to reflect the staining patterns likely to be seen in routine diagnostic practice. Positive staining refers to staining of neoplastic cells.
73% of giant cell glioblastomas showed some degree of staining for CD34, and 55% showed strong widespread staining. 56% of non-giant cell glioblastomas showed some degree of CD34 staining, and 25% showed strong widespread staining.
Both giant cell and non-giant cell glioblastomas frequently show CD34 expression by neoplastic cells, which may in some cases be strong and diffuse. Strong widespread staining of neoplastic cells for CD34 was more frequent in giant cell than non-giant cell glioblastomas, however this difference was not statistically significant. CD34 staining in isolation is unlikely to be of assistance in differentiating between giant cell glioblastoma and pleomorphic xanthoastrocytomas with anaplastic features or anaplastic gangliogliomas.
本研究旨在确定CD34是否在胶质母细胞瘤和巨细胞胶质母细胞瘤中表达，因为在试图区分巨细胞胶质母细胞瘤与其他相关鉴别诊断（如具有间变特征的多形性黄色星形细胞瘤和间变性节细胞胶质瘤）时，该信息可能具有价值。
对11例巨细胞胶质母细胞瘤和16例非巨细胞胶质母细胞瘤进行CD34免疫细胞化学染色评估。
采用标准免疫细胞化学技术，以反映常规诊断实践中可能出现的染色模式。阳性染色指肿瘤细胞染色。
73%的巨细胞胶质母细胞瘤显示出一定程度的CD34染色，55%显示出强烈的广泛染色。56%的非巨细胞胶质母细胞瘤显示出一定程度的CD34染色，25%显示出强烈的广泛染色。
巨细胞和非巨细胞胶质母细胞瘤的肿瘤细胞均经常显示CD34表达，在某些情况下可能是强烈且弥漫性的。与非巨细胞胶质母细胞瘤相比，巨细胞胶质母细胞瘤中肿瘤细胞CD34的强烈广泛染色更为常见，但这种差异无统计学意义。单独的CD34染色不太可能有助于区分巨细胞胶质母细胞瘤与具有间变特征的多形性黄色星形细胞瘤或间变性节细胞胶质瘤。
