Division of Virology, Department of Microbiology and Immunology, Osaka University Graduate School of Medicine, 2-2 Yamada-oka, Suita, Osaka 565-0871, Japan.
Biochem Biophys Res Commun. 2010 Apr 9;394(3):482-7. doi: 10.1016/j.bbrc.2010.02.122. Epub 2010 Feb 20.
We analyzed the gene expression profiles of lymphocyte-originated tumor cell lines - primary effusion lymphoma (PEL) cell lines, T-cell leukemia (TCL) cell lines, Burkitt lymphoma (BL) cell lines - and two sets of normal peripheral blood mononuclear cells (PBMCs) - in order to determine characteristic gene expression profiles for each of the former three groups. And we found that these cell lines showed respective typical gene expression profiles and classified into clear four groups, PEL, TCL, BL, and normal PBMCs. Two B lymphocyte-originated tumor cell lines, PEL and BL cell lines, clearly exhibited distinct gene expression profiles, respectively. Even though there was only one line that was co-infected with both Kaposi's sarcoma-associated herpesvirus (KSHV) and Epstein-Barr virus (EBV), KSHV seemed to govern the gene expression profile of the co-infected line. These data suggested not only that established typical tumor cell lines show a distinct gene expression profile but also that this profile may be governed by certain viruses.
我们分析了源自淋巴细胞的肿瘤细胞系——原发性渗出性淋巴瘤（PEL）细胞系、T 细胞白血病（TCL）细胞系、伯基特淋巴瘤（BL）细胞系——以及两组正常外周血单核细胞（PBMC）的基因表达谱，以确定前三个组各自的特征基因表达谱。我们发现这些细胞系表现出各自典型的基因表达谱，并分为四个明确的组，即 PEL、TCL、BL 和正常 PBMC。两种 B 淋巴细胞来源的肿瘤细胞系，PEL 和 BL 细胞系，分别表现出明显不同的基因表达谱。尽管只有一条线同时感染了卡波济肉瘤相关疱疹病毒（KSHV）和 Epstein-Barr 病毒（EBV），但 KSHV 似乎控制了共感染线的基因表达谱。这些数据不仅表明已建立的典型肿瘤细胞系表现出独特的基因表达谱，而且该谱可能受某些病毒的控制。
