Key Laboratory for AgroBiotecnology of Shihezi University, Shihezi, Xinjiang 832003, PR China.
Antiviral Res. 2010 Aug;87(2):265-8. doi: 10.1016/j.antiviral.2010.02.319. Epub 2010 Feb 20.
We have constructed 2 small interfering RNAs (siRNAs) specifically targeting homogenous 3D and 2B1 regions of 7 serotypes of the foot and mouth disease virus (FMDV) and tested the ability of siRNAs to inhibit virus replication in baby hamster kidney (BHK-21) cells and suckling mice. In this study, we generated transgenic mouse models integrating short hairpin RNA (shRNA) targeting microinfected FMDV. When examined at the 7th passage in transgenic mice, the target gene was still found by PCR to be integrated in the genome. Compared to the control mice, the transgenic mice showed only slightly abnormal pathology when they were infected with the FMDV serotype Asia 1. The number of viruses in the tissues of the transgenic mouse was very low and in some tissues no virus could be detected by immunohistochemistry.
我们构建了 2 种针对口蹄疫病毒（FMDV） 7 种血清型同源 3D 和 2B1 区的小干扰 RNA（siRNA），并测试了 siRNA 抑制 BHK-21 细胞和乳鼠中病毒复制的能力。在这项研究中，我们构建了整合靶向 microinfected FMDV 的短发夹 RNA（shRNA）的转基因小鼠模型。在转基因小鼠的第 7 代进行检测时，PCR 仍发现靶基因整合到基因组中。与对照小鼠相比，当感染 FMDV 血清型 Asia 1 时，转基因小鼠仅表现出轻微的异常病理学。转基因小鼠组织中的病毒数量非常低，在某些组织中，免疫组织化学检测不到病毒。
