Molecular Cell Physiology & Endocrinology, Institute for Zoology, Technische Universität Dresden, Dresden, Germany.
Mol Cell Endocrinol. 2010 Jun 10;321(2):253-7. doi: 10.1016/j.mce.2010.02.018. Epub 2010 Feb 20.
Several authors have investigated the role of aryl hydrocarbon receptor (AHR) in the reproductive tract, but there are no data available whether 17beta-estradiol (E2) regulates expression of members of the AHR pathway in the uterus. We therefore examined the mRNA expression of Ahr as well as the genes of the AHR dimerization partners ARNT1 and ARNT2 and the AHR regulated genes Cyp1a1 and Gsta2 in the uterus of ovariectomized rats after administration of E2 at two different doses. The data show that Ahr mRNA expression is downregulated while AHR protein amounts increased in all uterine tissue compartments. In addition we observed a downregulation of Arnt1, Arnt2 and Cyp1a1 while Gsta2 mRNA expression is upregulated by E2 in a dose-dependent manner. These results show that members of the AHR pathway are regulated by E2 in the uterus. AHR may therefore play an important role in the mediation of uterine estrogenic effects.
几位作者研究了芳香烃受体（AHR）在生殖道中的作用，但目前尚无数据表明 17β-雌二醇（E2）是否调节子宫中 AHR 途径成员的表达。因此，我们在给予两种不同剂量的 E2 后，检查了子宫中 Ahr 以及 AHR 二聚化伴侣 ARNT1 和 ARNT2 的基因和 AHR 调节基因 Cyp1a1 和 Gsta2 的 mRNA 表达。数据表明，Ahr mRNA 表达下调，而 AHR 蛋白含量在所有子宫组织区室中增加。此外，我们观察到 Arnt1、Arnt2 和 Cyp1a1 的下调，而 Gsta2 mRNA 表达则呈剂量依赖性上调。这些结果表明，AHR 途径的成员受 E2 在子宫中的调节。因此，AHR 可能在介导子宫雌激素效应中发挥重要作用。
