Korea Institute of Science and Technology - Europe Forschungsges. mbH Saarbücken, Germany.
Exp Mol Med. 2010 May 31;42(5):319-26. doi: 10.3858/emm.2010.42.5.030.
To identify immunoglobulin variable heavy chain (VH) gene usages in Korean ankylosing spondylitis (AS) patients, expression level of VH2 genes from peripheral blood mononuclear cells (PBMCs) of 8 AS patients and 9 healthy donors was analysed by quantitative real-time PCR (Q-PCR). Q-PCR results demonstrated VH2 genes were overexpressed in AS patients (Relative amount of mRNA of VH2 genes to a house-keeping gene, 7.13+/-7.77 vs, 0.68+/-0.55; P<0.0001). The sequence analysis revealed the majority of them contained CDC42 binding protein kinase Beta (CDC42 BPB) genes. The insertion of CDC42 BPB gene was confirmed by PCR with primers corresponding CDC42 BPB and CH genes. Our study revealed VH2 overexpression and unique rearrangement in Ig VH genes from peripheral blood of AS patients. This may imply aberrant immunoglobulin gene rearrangement in B cell occurs in Korean AS patients, which requires further investigation.
为了鉴定韩国强直性脊柱炎(AS)患者的免疫球蛋白重链可变区(VH)基因使用情况,通过定量实时 PCR(Q-PCR)分析了 8 名 AS 患者和 9 名健康供体外周血单个核细胞(PBMC)中 VH2 基因的表达水平。Q-PCR 结果表明,AS 患者中 VH2 基因过度表达(VH2 基因相对于管家基因的 mRNA 相对量,7.13+/-7.77 对 0.68+/-0.55;P<0.0001)。序列分析表明,它们中的大多数含有 CDC42 结合蛋白激酶 Beta(CDC42 BPB)基因。使用与 CDC42 BPB 和 CH 基因相对应的引物通过 PCR 证实了 CDC42 BPB 基因的插入。我们的研究揭示了 AS 患者外周血中 VH2 的过度表达和独特重排。这可能意味着韩国 AS 患者的 B 细胞中发生了异常的免疫球蛋白基因重排,这需要进一步研究。