Joshi Maithilee, Nikalje A P, Shahed M, Dehghan M
Y. B. Chavan College of Pharmacy, Dr. Rafiq Zakaria Campus, Rauza Bagh, Auranagabad-431 001, India.
Indian J Pharm Sci. 2009 Jan;71(1):95-7. doi: 10.4103/0250-474X.51951.
A simple, precise, accurate and rapid high performance thin layer chromatographic method has been developed and validated for the estimation of emtricitabine and tenofovir simultaneously in combined dosage form. The stationary phase used was precoated silica gel 60F 254. The mobile phase used was a mixture of chloroform: methanol (9:1 v/v). The detection of spots was carried out at 265 nm. The method was validated in terms of linearity, accuracy, precision and specificity. The calibration curve was found to be linear between 200 to 1000 ng with regression coefficient of 0.9995. The proposed method can be successfully used to determine the drug content of marketed tablet formulation.
已开发并验证了一种简单、精确、准确且快速的高效薄层色谱法，用于同时测定复方制剂中恩曲他滨和替诺福韦的含量。所用固定相为预涂硅胶60F 254。所用流动相为氯仿：甲醇（9:1 v/v）的混合物。斑点检测在265 nm波长下进行。该方法在线性、准确度、精密度和特异性方面进行了验证。校准曲线在200至1000 ng之间呈线性，回归系数为0.9995。所提出的方法可成功用于测定市售片剂制剂的药物含量。
