Shinde P B, Aragade P D, Agrawal M R, Deokate U A, Khadabadi S S
Sitabai Thite College of Pharmacy, Shirur, Pune-412 210, India.
Indian J Pharm Sci. 2011 Mar;73(2):240-3. doi: 10.4103/0250-474x.91573.
The objective of this work was to develop and validate a simple, rapid, precise, and accurate high performance thin layer chromatography method for simultaneous determination of withanolide A and bacoside A in combined dosage form. The stationary phase used was silica gel G60F(254). The mobile phase used was mixture of ethyl acetate: methanol: toluene: water (4:1:1:0.5 v/v/v/v). The detection of spots was carried out at 320 nm using absorbance reflectance mode. The method was validated in terms of linearity, accuracy, precision and specificity. The calibration curve was found to be linear between 200 to 800 ng/spot for withanolide A and 50 to 350 ng/spot for bacoside A. The limit of detection and limit of quantification for the withanolide A were found to be 3.05 and 10.06 ng/spot, respectively and for bacoside A 8.3 and 27.39 ng/spot, respectively. The proposed method can be successfully used to determine the drug content of marketed formulation.
本研究的目的是开发并验证一种简单、快速、精确且准确的高效薄层色谱法，用于同时测定复方制剂中睡茄内酯A和积雪草苷A的含量。所用固定相为硅胶G60F(254)。所用流动相为乙酸乙酯:甲醇:甲苯:水(4:1:1:0.5 v/v/v/v)的混合物。采用吸光度反射模式在320 nm处进行斑点检测。该方法在线性、准确度、精密度和特异性方面进行了验证。发现睡茄内酯A的校准曲线在200至800 ng/斑点之间呈线性，积雪草苷A的校准曲线在50至350 ng/斑点之间呈线性。睡茄内酯A的检测限和定量限分别为3.05和10.06 ng/斑点，积雪草苷A的检测限和定量限分别为8.3和27.39 ng/斑点。所提出的方法可成功用于测定市售制剂的药物含量。
