2,4-二氯苯氧乙酸对中国仓鼠卵巢细胞中多胺合成的影响。

Effects of 2,4-dichlorophenoxyacetic acid on polyamine synthesis in Chinese hamster ovary cells.

作者信息

Rivarola V A, Balegno H F

机构信息

Departamento de Biología Molecular, Universidad Nacional de Río Cuarto, Córdoba, Argentina.

出版信息

Toxicol Lett. 1991 Apr;56(1-2):151-7. doi: 10.1016/0378-4274(91)90101-b.

DOI:10.1016/0378-4274(91)90101-b

PMID:2017772

Abstract

It was found that 1 mM 2,4-dichlorophenoxyacetate (2,4-D) inhibited DNA and protein synthesis in Chinese hamster ovary (CHO) cells. When the possible relationship of this phenomenon to the presence of polyamines in the culture medium was investigated, it was found that: (a) the pesticide inhibited ornithine decarboxylase activity; (b) when the concentration of polyamines present in cells treated with the pesticide was determined, the putrescine concentration did not change, and the spermine and spermidine concentration decreased; (c) the addition of spermidine and spermine to CHO cells grown in the presence of 2,4-D normalized DNA and protein synthesis. Putrescine did not have any effect.

摘要

研究发现，1毫摩尔的2,4 - 二氯苯氧乙酸（2,4 - D）可抑制中国仓鼠卵巢（CHO）细胞中的DNA和蛋白质合成。当研究这种现象与培养基中多胺存在的可能关系时，发现：（a）该农药抑制鸟氨酸脱羧酶活性；（b）测定用该农药处理的细胞中多胺的浓度时，腐胺浓度不变，精胺和亚精胺浓度降低；（c）向在2,4 - D存在下生长的CHO细胞中添加亚精胺和精胺可使DNA和蛋白质合成恢复正常。腐胺没有任何作用。