Department of Biology, Biotechnical Faculty, University of Ljubljana, Vecna pot 111, 1000, Ljubljana, Slovenia.
Protoplasma. 2010 Oct;246(1-4):33-9. doi: 10.1007/s00709-010-0117-9. Epub 2010 Feb 23.
Oogenesis in the neotenic, cave dwelling salamander Proteus anguinus anguinus has not been studied yet, and this study provides a detailed description of the early growth of the oocytes. Early previtellogene oocytes ranging from 100 to 600 µm in diameter were examined by light and transmission electron microscopy. The oocytes were divided into two stages based on size, color, and histology. Stage I oocytes can be identified by their transparent cytoplasm and a homogenous juxtanuclear mass, composed of numerous lipid droplets and mitochondria. Stage II oocytes are no longer transparent and have increased in diameter to 300- 600 µm, and many cortical alveoli differing in size have appeared. The common and most predominant ultrastructural characteristics of both stages of previtellogene oocytes are extensive quantities of smooth membrane, numerous mitochondria, and lipid droplets, as well as abundant free ribosomes. Myeline-like structures and remarkable annulate lamellae of closely packed membrane stacks are also frequently observed. Previtellogenic oocytes are the most predominant oocytes in the ovaries of Proteus, and while they possess certain structural characteristics typical for other amphibians, some features are unique and could result from adaptation to the subterranean environment.
尚未对洞穴居住的蝾螈 Proteus anguinus anguinus 的卵母细胞发生进行研究，本研究详细描述了卵母细胞的早期生长情况。通过光镜和透射电镜检查了直径在 100 到 600μm 之间的早期前卵母细胞。根据大小、颜色和组织学特征，将卵母细胞分为两个阶段。I 期卵母细胞可通过透明的细胞质和均匀的核周质团来识别，核周质团由许多脂滴和线粒体组成。II 期卵母细胞不再透明，直径增加到 300-600μm，并且出现了许多大小不同的皮质小泡。两个前卵母细胞阶段的共同且最主要的超微结构特征是大量的光滑膜、许多线粒体和脂滴，以及丰富的游离核糖体。髓磷脂样结构和紧密堆积的膜堆叠的明显环状层板也经常观察到。前卵母细胞是 Proteus 卵巢中最主要的卵母细胞，虽然它们具有其他两栖动物的某些典型结构特征，但有些特征是独特的，可能是适应地下环境的结果。
