Department of Genetics & Development, Columbia University Medical Center, 701 West 168th Street, HHSC 1608, New York, NY 10032-2704, USA.
Cell Div. 2010 Feb 23;5:7. doi: 10.1186/1747-1028-5-7.
Regulation of the repair of DNA double-strand breaks by homologous recombination is extremely important for both cell viability and the maintenance of genomic integrity. Modulation of double-strand break repair in the yeast Saccharomyces cerevisiae involves controlling the recruitment of one of the central recombination proteins, Rad52, to sites of DNA lesions. The Rad52 protein, which plays a role in strand exchange and the annealing of single strand DNA, is positively regulated upon entry into S phase, repressed during the intra-S phase checkpoint, and undergoes posttranslational modification events such as phosphorylation and sumoylation. These processes all contribute to the timing of Rad52 recruitment, its stability and function. Here, we summarize the regulatory events affecting the Rad52 protein and discuss how this regulation impacts DNA repair and cell survival.
同源重组对 DNA 双链断裂的修复调控对于细胞活力和基因组完整性的维持至关重要。在酿酒酵母中，调节双链断裂修复涉及控制中央重组蛋白之一 Rad52 蛋白招募到 DNA 损伤部位。Rad52 蛋白在链交换和单链 DNA 的退火中发挥作用，在进入 S 期时被正向调控，在 S 期内检验点期间受到抑制，并经历翻译后修饰事件，如磷酸化和 SUMO 化。这些过程都有助于 Rad52 蛋白的募集、稳定性和功能的时间调控。本文总结了影响 Rad52 蛋白的调控事件，并讨论了这种调控如何影响 DNA 修复和细胞存活。
