Laboratorio de Psicobiología, Universidad de Sevilla, Sevilla, Spain.
Behav Brain Res. 2010 Jul 11;210(2):191-201. doi: 10.1016/j.bbr.2010.02.031. Epub 2010 Feb 21.
The involvement of the main pallial subdivisions of the teleost telencephalic pallium in spatial cognition was evaluated in a series of three experiments. The first two compared the effects of lesions selective to the lateral (LP), medial (MP) and dorsal (DP) telencephalic pallium of goldfish, on the retention and the reversal learning of a spatial constancy task which requires the use of allocentric or relational strategies. The results showed that LP lesions produced a selective impairment on the capability of goldfish to solve the spatial task previously learned and on the reversal learning of the same procedure, whereas MP and DP lesions did not produce observable deficits. The third experiment evaluated, by means of the AgNOR stain, learning-dependent changes of the neuronal transcription activity in the pallium of goldfish trained in the spatial constancy task or in a cue version of the same procedure, which only differed on their spatial cognition demands. The results revealed that training in the spatial task produced an increment in the transcriptive activity which was selective to the neurons of the ventral lateral pallium, as indicated by increases in the size of the nucleolar organizing region (NOR), the nucleolar organelles associated with the synthesis of ribosomal proteins. In contrast, training in the cue version did not produced observable changes. These data, revealing a striking functional similarity between the lateral telencephalic pallium of the teleost fish and the amniote hippocampus, provide additional evidence regarding the homology of both structures.
在三个实验系列中评估了硬骨鱼端脑皮层的主要脑叶分支在空间认知中的作用。前两个实验比较了选择性损伤金鱼外侧（LP）、内侧（MP）和背侧（DP）端脑皮层对空间恒常性任务保留和反转学习的影响，该任务需要使用以自我为中心或关系策略。结果表明，LP 损伤选择性地损害了金鱼解决先前学习的空间任务的能力，以及对相同程序的反转学习能力，而 MP 和 DP 损伤则没有产生可观察到的缺陷。第三个实验通过 AgNOR 染色评估了在空间恒常性任务或相同程序的线索版本中受过训练的金鱼的脑皮层的学习依赖性转录活性变化，这两种程序仅在空间认知要求上有所不同。结果表明，在空间任务中的训练导致了转录活性的增加，这是腹外侧脑叶的神经元选择性的，如核仁组成区（NOR）的大小增加所表明的，这与核糖体蛋白合成相关的核仁细胞器。相比之下，在线索版本的训练中没有产生可观察到的变化。这些数据揭示了硬骨鱼端脑外侧脑叶与羊膜动物海马之间惊人的功能相似性，为这两种结构的同源性提供了额外的证据。
