Department of Neurosurgery, Zhujiang Hospital, Southern Medical University, Guangzhou 510282, China.
Neurosci Lett. 2010 Apr 12;473(3):186-91. doi: 10.1016/j.neulet.2010.02.035. Epub 2010 Feb 21.
Endothelial progenitor cells (EPCs) are responsible for postnatal vasculogenesis in physiological and pathological neovascularization. Adipose tissue (AT) is an abundant source of mesenchymal stem cells (MSCs), which have multipotent differentiation ability. We successfully derived EPCs from AT, which maintained a strong proliferative capacity and demonstrated the characteristic endothelial function of uptaking of acetylated low-density lipoprotein. They formed tube-like structures in vitro. Endothelial nitric oxide synthase (eNOS) gene expression in EPCs was similar to that in mature endothelial cells. Transplantation of EPCs derived from AT after the acute phase was applied in rats with traumatic brain injury (TBI). Transplanted EPCs participated in the neovascularization of injured brain. Improving functional recovery, reducement of deficiency volume of brain, host astrogliosis and inflammation were found. These results suggest that adult AT derived stem cells can be induced to functional EPCs and have beneficial effect on cell therapy.
内皮祖细胞（EPCs）负责生理和病理新生血管形成中的出生后血管生成。脂肪组织（AT）是间充质干细胞（MSCs）的丰富来源，具有多能分化能力。我们成功地从 AT 中衍生出 EPCs，它们保持着强大的增殖能力，并表现出摄取乙酰化低密度脂蛋白的特征性内皮功能。它们在体外形成管状结构。EPCs 中的内皮型一氧化氮合酶（eNOS）基因表达与成熟内皮细胞相似。在创伤性脑损伤（TBI）大鼠中应用急性后期来源于 AT 的 EPC 移植。移植的 EPC 参与了损伤大脑的新血管形成。发现功能恢复改善、脑缺损体积减少、宿主星形胶质细胞增生和炎症减轻。这些结果表明，成体 AT 来源的干细胞可以被诱导为功能性 EPCs，并对细胞治疗有有益的作用。
