Department of Oncology and Radiotherapy, Oulu University Hospital, Finland.
Br J Cancer. 2010 Mar 16;102(6):1018-23. doi: 10.1038/sj.bjc.6605565. Epub 2010 Feb 23.
8-Hydroxydeoxyguanosine (8-oxodG) is the commonly used marker of oxidative stress-derived DNA damage. 8-OxodG formation is regulated by local antioxidant capacity and DNA repair enzyme activity. Earlier studies have reported contradictory data on the function of 8-oxodG as a prognostic factor in different cancer types.
We assessed pre-operative serum 8-oxodG levels with an enzyme-linked immunosorbent assay in a well-defined series of 173 breast cancer patients. 8-OxodG expression in the nuclei of cancer cells from 150 of these patients was examined by immunohistochemistry.
The serum 8-oxodG levels and immunohistochemical 8-oxodG expression were in concordance with each other (P<0.05). Negative 8-oxodG immunostaining was an independent prognostic factor for poor breast cancer-specific survival according to the multivariate analysis (P<0.01). This observation was even more remarkable when ductal carcinomas only (n=140) were considered (P<0.001). A low serum 8-oxodG level was associated statistically significantly with lymphatic vessel invasion and a positive lymph node status.
Low serum 8-oxodG levels and a low immunohistochemical 8-oxodG expression were associated with an aggressive breast cancer phenotype. In addition, negative 8-oxodG immunostaining was a powerful prognostic factor for breast cancer-specific death in breast carcinoma patients.
8-羟基脱氧鸟苷（8-oxodG）是氧化应激衍生的 DNA 损伤的常用标志物。8-oxodG 的形成受局部抗氧化能力和 DNA 修复酶活性的调节。早期的研究报告了不同癌症类型中 8-oxodG 作为预后因素的功能的矛盾数据。
我们使用酶联免疫吸附试验（ELISA）在 173 例乳腺癌患者的明确系列中评估了术前血清 8-oxodG 水平。通过免疫组织化学检查了其中 150 例患者的癌细胞核中的 8-oxodG 表达。
血清 8-oxodG 水平和免疫组化 8-oxodG 表达彼此一致（P<0.05）。根据多变量分析，阴性 8-oxodG 免疫染色是乳腺癌特异性生存不良的独立预后因素（P<0.01）。当仅考虑导管癌（n=140）时，这一观察结果更为显著（P<0.001）。低血清 8-oxodG 水平与淋巴管侵犯和阳性淋巴结状态有统计学显著相关性。
低血清 8-oxodG 水平和低免疫组化 8-oxodG 表达与侵袭性乳腺癌表型相关。此外，阴性 8-oxodG 免疫染色是乳腺癌患者乳腺癌特异性死亡的有力预后因素。
