Mir I H, Channa A
Department of Zoology, University of Kashmir, Srinagar-190006, India.
Pak J Biol Sci. 2009 Dec 1;12(23):1511-5. doi: 10.3923/pjbs.2009.1511.1515.
Using the scanning electron microscope, the gills of Schizothorax curvifrons have been studied after carrying out their primary fixation in 2.5% glutaraldehyde in 0.1 M sodiumcacodylate buffer (pH 7.3) for 24 h at 0-4 degrees C and secondary fixation in 1-4% osmiumtetraoxide in 0.1 M sodiumcacodylate buffer (pH 7.3) for 1-2 h at 0-4 degrees C. The gills are observed to comprise of gill arch, gill rakers having minute projections on their inner sides and gill filaments. The filaments originating from the gill arch are in a linear fashion and equidistant from each other, bearing secondary lamellae on both sides of the filament axis and each lamellae running parallel to the adjacent lamellae. The filament epithelium is found to be equipped with pavement cells, mucous cells and chloride cells. The pavement cells have smooth surface with sparse and irregular microridges defining their cell limits. The mucous cells are mainly found on the leading and trailing edges of the filament and the chloride cells are located close to the onset of secondary lamellae. The possible roles of these structures and cells in relation to respiration, water flow and feeding are discussed, setting thereby a platform for further studies in relation to pathology, pollution and stress conditions in aquaculture, natural and polluted environments.
使用扫描电子显微镜，对弯口裂腹鱼的鳃进行了研究。鳃先在0.1 M二甲胂酸钠缓冲液（pH 7.3）中的2.5%戊二醛中于0-4℃固定24小时，然后在0.1 M二甲胂酸钠缓冲液（pH 7.3）中的1-4%四氧化锇中于0-4℃固定1-2小时。观察到鳃由鳃弓、内侧有微小突起的鳃耙和鳃丝组成。起源于鳃弓的鳃丝呈线性排列且彼此等距，在鳃丝轴的两侧有次生鳃小片，每个鳃小片与相邻鳃小片平行排列。发现鳃丝上皮配备有扁平细胞、黏液细胞和氯细胞。扁平细胞表面光滑，有稀疏且不规则的微嵴界定其细胞界限。黏液细胞主要见于鳃丝的前缘和后缘，氯细胞位于次生鳃小片起始处附近。讨论了这些结构和细胞在呼吸、水流和摄食方面可能的作用，从而为进一步研究水产养殖、自然和污染环境中的病理学、污染和应激条件搭建了一个平台。
