College of Agriculture and Bioresources, University of Saskatchewan, Saskatoon, SK, Canada.
J Agric Food Chem. 2010 Mar 24;58(6):3460-4. doi: 10.1021/jf904179m.
The objectives of this study were to determine the protein molecular structures of the new coproducts from bioethanol production, quantify protein structure amide I to II and alpha-helix to beta-sheet spectral peak intensity ratio, and illustrate multivariate molecular spectral analyses as a novel research tool for rapid characterization of protein molecular structures in bioethonal bioproducts. The study demonstrated that the grains had a significantly higher ratio of alpha-helix to beta-sheet in the protein structure than their coproducts produced from bioethanol processing (1.38 vs 1.03, P < 0.05). There were significant differences between wheat and corn (1.47 vs 1.29, P < 0.05) but no difference between wheat dried distiller grains with solubles (DDGS) and corn DDGS (1.04 vs 1.03, P > 0.05). The grains had a significantly higher ratio of protein amide I to II in the protein structure than their coproducts produced from bioethanol processing (4.58 vs 2.84, P < 0.05). There were no significant differences between wheat and corn (4.61 vs 4.56, P > 0.05), but there were significant differences between wheat DDGS and corn DDGS (3.08 vs 2.21, P < 0.05). This preliminary study indicated that bioethanol processing changes protein molecular structures, compared with original grains. Further study is needed with a large set of the new bioethanol coproducts to quantify protein molecular structures (alpha-helix to beta-sheet ratio; amide I to II ratio) of the bioethanol coproducts in relation to nutrient supply and availability in animals.
本研究旨在确定生物乙醇生产中新产品的蛋白质分子结构,定量测定蛋白质结构酰胺 I 到 II 和α-螺旋到β-折叠谱峰强度比,并说明多元分子光谱分析作为一种快速表征生物乙醇生物制品中蛋白质分子结构的新研究工具。研究表明,与生物乙醇加工生产的副产品相比,谷物的蛋白质结构中α-螺旋到β-折叠的比例明显更高(1.38 对 1.03,P < 0.05)。小麦和玉米之间存在显著差异(1.47 对 1.29,P < 0.05),但小麦干燥酒糟和可溶物(DDGS)与玉米 DDGS 之间没有差异(1.04 对 1.03,P > 0.05)。与生物乙醇加工生产的副产品相比,谷物的蛋白质结构中酰胺 I 到 II 的比例明显更高(4.58 对 2.84,P < 0.05)。小麦和玉米之间没有显著差异(4.61 对 4.56,P > 0.05),但小麦 DDGS 和玉米 DDGS 之间存在显著差异(3.08 对 2.21,P < 0.05)。本初步研究表明,与原谷物相比,生物乙醇加工会改变蛋白质的分子结构。需要进一步研究大量的新型生物乙醇副产品,以定量测定生物乙醇副产品的蛋白质分子结构(α-螺旋到β-折叠的比例;酰胺 I 到 II 的比例)与动物营养供应和可用性的关系。