Department of Obstetrics and Gynecology, Sherbrooke University Hospital Centre, 3001, 12e Avenue Nord, Sherbrooke, Quebec J1H 5N4, Canada.
J Ovarian Res. 2010 Jan 28;3:3. doi: 10.1186/1757-2215-3-3.
Endometrioid carcinoma of the ovary is one of the most types of epithelial ovarian cancer associated to endometrioisis. Endometrioid tumors as well as endometriotic implants are characterized by the presence of epithelial cells, stromal cells, or a combination of booth, that resemble the endometrial cells, suggesting a possible endometrial origin of these tumors. Pro-inflammatory cytokines, including interleukin-1 (IL-1) have been reported to be involved in both endometriosis and ovarian carcinogenesis. The major objective of this study was to determine the level expression of IL-1 ligands system (IL-1alpha, IL-1beta and IL-1RA) in the most common subtypes of ovarian cancer cells compared to endometrial cells.
We used primary endometrial cells, endometrial cell line RL-952 and different subtypes of epithelial ovarian cancer cell lines including TOV-112D (endometrioid), TOV-21G (clear cell) and OV-90 (serous). Immunofluorescence and real-time PCR analysis were used respectively for detecting IL-1 ligands at the levels of cell-associated protein and mRNA. Soluble IL-1 ligands were analyzed by ELISA.
We demonstrated that IL-1 ligands were expressed by all endometriosis-associated ovarian cancer subtypes and endometrial cells. In contrast to other cancer ovarian cells, endometrioid cells exhibit a specific decrease of cell-associated IL-1RA expression and its soluble secretion.
Endometrioid ovarian cancer exhibits an alteration in the expression of IL-1RA, a key protector against tumorogenic effects of IL-1. This alteration evokes the same alteration observed in endometriotic cells in previous studies. This suggests a possible link between the endometrium, the tissue ectopic endometriosis and endometrioid ovarian cancer.
卵巢子宫内膜样癌是与子宫内膜异位症相关的上皮性卵巢癌的最常见类型之一。子宫内膜样肿瘤和子宫内膜异位症种植体的特征是存在上皮细胞、基质细胞或两者的组合，类似于子宫内膜细胞，提示这些肿瘤可能来自子宫内膜。促炎细胞因子，包括白细胞介素-1（IL-1），已被报道参与子宫内膜异位症和卵巢癌的发生。本研究的主要目的是确定与子宫内膜细胞相比，在最常见的卵巢癌细胞亚型中 IL-1 配体系统（IL-1alpha、IL-1beta 和 IL-1RA）的表达水平。
我们使用原代子宫内膜细胞、子宫内膜细胞系 RL-952 和不同的上皮性卵巢癌细胞系，包括 TOV-112D（子宫内膜样）、TOV-21G（透明细胞）和 OV-90（浆液性）。分别采用免疫荧光和实时 PCR 分析检测细胞相关蛋白和 mRNA 水平的 IL-1 配体。通过 ELISA 分析可溶性 IL-1 配体。
我们证明了 IL-1 配体在上皮性卵巢癌的所有子宫内膜异位症相关亚型和子宫内膜细胞中均有表达。与其他卵巢癌细胞不同，子宫内膜样细胞表现出细胞相关 IL-1RA 表达及其可溶性分泌的特异性降低。
子宫内膜样卵巢癌表现出 IL-1RA 表达的改变，IL-1RA 是一种对抗 IL-1 致瘤作用的关键保护因子。这种改变与之前研究中观察到的子宫内膜异位症细胞中的改变相同。这表明子宫内膜、异位的子宫内膜组织和子宫内膜样卵巢癌之间可能存在联系。
