Beytut E
Department of Pathology, Faculty of Veterinary Medicine, University of Kafkas, Kars, Turkey.
J Comp Pathol. 2010 Aug-Oct;143(2-3):132-41. doi: 10.1016/j.jcpa.2010.01.014. Epub 2010 Feb 23.
The present study investigated the expression of pulmonary surfactant proteins (surfactant protein [SP]-A, SP-B, proSP-C), thyroid transcription factor-1 (TTF-1), proliferating cell nuclear antigen (PCNA) and the infiltration of CD3(+) T and CD79alphacy(+) B lymphocytes into the lungs of 10 lambs naturally infected with sheep pox virus. Microscopical examination detected marked epithelial hyperplasia, sheep pox cells and neutrophilic infiltration in pock nodules. Immunohistochemistry demonstrated intense expression of SPs in the cytoplasm of the hyperplastic type II pneumocytes in the nodular pock lesions. These cells displayed a gland-like arrangement. The hyperplastic and the normal bronchiolar epithelium did not express SPs. The nuclei of the hyperplastic and normal type II pneumocytes labelled positively for TTF-1. Strong PCNA positivity indicated epithelial cell proliferation in the pock nodules. Moderate to abundant numbers of CD3(+) T cells, but few CD79alphacy B lymphocytes, were detected in the pock lesions. Transmission electron microscopy revealed that the hyperplasia in the pock nodules comprised predominantly type II pneumocytes and that the sheep pox cells and epithelial cells contained virus particles in their cytoplasm. The results of this study show that sheep pox virus induces marked proliferation of type II pneumocytes and bronchiolar epithelial cells and that the lung lesions in diseased lambs are mainly proliferative.
本研究调查了10只自然感染羊痘病毒的羔羊肺组织中肺表面活性物质蛋白（表面活性蛋白[SP]-A、SP-B、前SP-C）、甲状腺转录因子-1（TTF-1）、增殖细胞核抗原（PCNA）的表达，以及CD3(+) T淋巴细胞和CD79αcy(+) B淋巴细胞在肺组织中的浸润情况。显微镜检查发现痘疹结节中有明显的上皮增生、羊痘细胞和嗜中性粒细胞浸润。免疫组织化学显示，结节性痘疹病变中增生的II型肺细胞胞质内有强烈的表面活性物质蛋白表达。这些细胞呈腺样排列。增生的和正常的细支气管上皮均不表达表面活性物质蛋白。增生的和正常的II型肺细胞的细胞核TTF-1染色呈阳性。PCNA强阳性表明痘疹结节中有上皮细胞增殖。在痘疹病变中检测到中等数量至大量的CD3(+) T细胞，但CD79αcy B淋巴细胞数量很少。透射电子显微镜显示，痘疹结节中的增生主要由II型肺细胞组成，羊痘细胞和上皮细胞的细胞质中含有病毒颗粒。本研究结果表明，羊痘病毒可诱导II型肺细胞和细支气管上皮细胞显著增殖，患病羔羊的肺部病变主要为增殖性病变。
