Department of Molecular Genetics and Microbiology, University of Massachusetts Medical School, 55 Lake Avenue North, Worcester, MA 01655, USA.
J Virol. 2010 May;84(9):4513-23. doi: 10.1128/JVI.01931-09. Epub 2010 Feb 24.
Virus-like particles (VLPs) released from avian cells expressing the Newcastle disease virus (NDV) strain AV proteins NP, M, HN (hemagglutinin-neuraminidase), and F were characterized. The VLP-associated HN and F glycoproteins directed the attachment of VLPs to cell surfaces and fusion of VLP membranes with red blood cell membranes, indicating that they were assembled into VLPs in an authentic conformation. These particles were quantitatively prepared and used as an immunogen, without adjuvant, in BALB/c mice. The resulting immune responses, detected by enzyme-linked immunosorbent assay (ELISA), virus neutralization, and intracellular cytokine staining, were comparable to the responses to equivalent amounts of inactivated NDV vaccine virus. HN and F proteins from another strain of NDV, strain B1, could be incorporated into these VLPs. Foreign peptides were incorporated into these VLPs when fused to the NP or HN protein. The ectodomain of a foreign glycoprotein, the Nipah virus G protein, fused to the NDV HN protein cytoplasmic and transmembrane domains was incorporated into ND VLPs. Thus, ND VLPs are a potential NDV vaccine candidate. They may also serve as a platform to construct vaccines for other pathogens.
从表达新城疫病毒（NDV）株 AV 蛋白 NP、M、HN（血凝素-神经氨酸酶）和 F 的禽类细胞中释放出的病毒样颗粒（VLPs）进行了表征。VLP 相关的 HN 和 F 糖蛋白指导 VLP 附着在细胞表面和 VLP 膜与红细胞膜融合，表明它们以真实构象组装成 VLPs。这些颗粒被定量制备并用作免疫原，无需佐剂，在 BALB/c 小鼠中使用。通过酶联免疫吸附测定（ELISA）、病毒中和和细胞内细胞因子染色检测到的免疫反应与等量的灭活 NDV 疫苗病毒的反应相当。来自另一种 NDV 株 B1 的 HN 和 F 蛋白可以掺入这些 VLPs 中。当融合到 NP 或 HN 蛋白时，外源肽被掺入这些 VLPs 中。融合到 NDV HN 蛋白胞质和跨膜结构域的外源糖蛋白尼帕病毒 G 蛋白的外显子被掺入 ND VLPs 中。因此，NDV VLPs 是一种潜在的 NDV 疫苗候选物。它们也可能作为构建针对其他病原体疫苗的平台。
