The Key Laboratory of Gene Engineering of the Ministry of Education, School of Life Science, Sun Yat-sen (Zhongshan) University, 510275 Guangzhou, China.
Mol Biol Rep. 2011 Jun;38(5):3189-94. doi: 10.1007/s11033-010-9990-8. Epub 2010 Feb 24.
An auxin response factor 2 gene, MiARF2, was cloned in our previous study [1] from the cotyledon section of mango (Mangifera indica L. cv. Zihua) during adventitious root formation, which shares an 84% amino acid sequence similarity to Arabidopsis ARF2. This study was to examine the effects of over-expression of the full-length MiARF2 open reading frame on the root and hypocotyl growth in Arabidopsis. Phenotype analysis showed that the T(3) transgenic lines had about 20-30% reduction in the length of hypocotyls and roots of the seedlings in comparison with the wild-type. The transcription levels of ANT and ARGOS genes which play a role in controlling organ size and cell proliferation in the transgenic seedlings also decreased. Therefore, the inhibited root and hypocotyl growth in the transgenic seedlings may be associated with the down-regulated transcription of ANT and ARGOS by the over-expression of MiARF2. This study also suggests that although MiARF2 only has a single DNA-binding domain (DBD), it can function as other ARF-like proteins containing complete DBD, middle region (MR) and carboxy-terminal dimerization domain (CTD).
在我们之前的研究中[1]，从芒果（Mangifera indica L. cv. Zihua）子叶部分克隆了一个生长素响应因子 2 基因 MiARF2，它与拟南芥 ARF2 的氨基酸序列相似性为 84%。本研究旨在研究全长 MiARF2 开放阅读框过表达对拟南芥根和下胚轴生长的影响。表型分析表明，与野生型相比，T(3)转基因株系的下胚轴和幼苗根长减少了约 20-30%。在转基因幼苗中，也降低了在控制器官大小和细胞增殖中起作用的 ANT 和 ARGOS 基因的转录水平。因此，转基因幼苗中根和下胚轴生长受到抑制可能与 MiARF2 的过表达下调 ANT 和 ARGOS 的转录有关。本研究还表明，尽管 MiARF2 仅具有单个 DNA 结合结构域（DBD），但它可以像其他含有完整 DBD、中间区域（MR）和羧基末端二聚化结构域（CTD）的 ARF 样蛋白一样发挥作用。
