Key Laboratory of Horticultural Plant Growth, Development and Quality Improvement, Ministry of Agricultural, Department of Horticulture, Zhejiang University, Hangzhou, 310058, China.
Zhejiang Provincial Key Laboratory of Horticultural Plant Integrative Biology, Zhejiang University, Hangzhou, 310058, China.
Sci Rep. 2018 Feb 14;8(1):2971. doi: 10.1038/s41598-018-21315-y.
Auxin response factors (ARFs) encode transcriptional factors that function in the regulation of plant development processes. A tomato ARF gene, SlARF5, was observed to be expressed at high levels in emasculated ovaries but maintained low expression levels in pollinated ovaries. The amiRNA SlARF5 lines exhibited ovary growth and formed seedless fruits following emasculation. These parthenocarpic fruits developed fewer locular tissues, and the fruit size and weight were decreased in transgenic lines compared to those of wild-type fruits. Gene expression analysis demonstrated that several genes involved in the auxin-signaling pathway were downregulated, whereas some genes involved in the gibberellin-signaling pathway were enhanced by the decreased SlARF5 mRNA levels in transgenic plants, indicating that SlARF5 may play an important role in regulating both the auxin- and gibberellin-signaling pathways during fruit set and development.
生长素响应因子（ARFs）编码转录因子，在植物发育过程的调控中发挥作用。番茄 ARF 基因 SlARF5 在去雄的子房中有高水平的表达，但在授粉的子房中表达水平较低。amiRNA SlARF5 株系在去雄后表现出子房生长和形成无籽果实。这些孤雌生殖的果实形成的腔室组织较少，与野生型果实相比，转基因株系的果实大小和重量都有所降低。基因表达分析表明，参与生长素信号通路的几个基因下调，而参与赤霉素信号通路的一些基因则由于转基因植物中 SlARF5 mRNA 水平的降低而增强，表明 SlARF5 在果实形成和发育过程中调节生长素和赤霉素信号通路中可能发挥重要作用。
