Frenz D A, Van de Water T R, Galinovíc-Schwartz V
Department of Otolaryngology, Rose F. Kennedy Center for Research in Mental Retardation and Human Development, Albert Einstein College of Medicine, Bronx, NY 10461.
Ann Otol Rhinol Laryngol. 1991 Apr;100(4 Pt 1):301-7. doi: 10.1177/000348949110000407.
Interactions between the epithelium of the otocyst and surrounding periotic mesenchyme direct the formation of the capsule of the mammalian inner ear. In the present study, we have characterized the temporal-spatial distribution of transforming growth factor beta 1 (TGF-beta 1) in the epithelial and mesenchymal tissues that compose the inner ear between 10 and 14 days of embryonic development. In addition, using high-density cultures of periotic mesenchyme to model otic capsule formation, we have demonstrated that exogenous TGF-beta 1 can modulate otic chondrogenesis by acting as either an enhancer or a suppressor of this process. Our immunohistochemical and in vitro results suggest a paracrine action for this growth factor in modulation of epithelial-mesenchymal tissue interactions and otic morphogenesis.
耳囊上皮与周围耳周间充质之间的相互作用指导了哺乳动物内耳囊的形成。在本研究中，我们已对胚胎发育第10至14天构成内耳的上皮和间充质组织中转化生长因子β1（TGF-β1）的时空分布进行了表征。此外，利用耳周间充质的高密度培养来模拟耳囊形成，我们已证明外源性TGF-β1可通过作为该过程的增强剂或抑制剂来调节耳软骨生成。我们的免疫组织化学和体外研究结果表明，这种生长因子在调节上皮-间充质组织相互作用和耳形态发生中具有旁分泌作用。
