Institute of Organic Chemistry, University of Würzburg, Am Hubland, 97074, Würzburg, Germany,
Adv Biochem Eng Biotechnol. 2010;120:163-93. doi: 10.1007/10_2009_54.
The integration of all relevant tools for bioreaction engineering has been a recent challenge. This approach should notably favor the production of oligo- and polysaccharides, which is highly complex due to the requirements of regio- and stereoselectivity. Oligosaccharides (OS) and polysaccharides (PS) have found many interests in the fields of food, pharmaceuticals, and cosmetics due to different specific properties. Food, sweeteners, and food ingredients represent important sectors where OS are used in major amounts. Increasing attention has been devoted to the sophisticated roles of OS and glycosylated compounds, at cell or membrane surfaces, and their function, e.g., in infection and cancer proliferation. The challenge for synthesis is obvious, and convenient approaches using cheap and readily available substrates and enzymes will be discussed. We report on new routes for the synthesis of oligosaccharides (OS), with emphasis on enzymatic reactions, since they offer unique properties, proceeding highly regio- and stereoselective in water solution, and providing for high yields in general.
近年来，将所有相关的生物反应工程工具集成一直是一个挑战。这种方法特别有利于寡糖和多糖的生产，由于区域和立体选择性的要求，这是非常复杂的。由于不同的特殊性质，寡糖 (OS) 和多糖 (PS) 在食品、制药和化妆品领域引起了广泛的关注。食品、甜味剂和食品配料是 OS 大量使用的重要领域。人们越来越关注 OS 和糖基化化合物在细胞或膜表面的复杂作用，以及它们的功能，例如在感染和癌症增殖中的作用。合成的挑战是显而易见的，将讨论使用廉价易得的底物和酶的便捷方法。我们报告了合成寡糖 (OS) 的新途径，重点是酶反应，因为它们提供了独特的性质，在水溶液中高度区域和立体选择性地进行，并且通常可以获得高产率。
