Center for Food Safety-IFSE, University of Arkansas, Fayetteville, Arkansas, USA.
J Environ Sci Health B. 2009 Aug;44(6):571-7. doi: 10.1080/03601230903000594.
A total of 10 ciprofloxacin-sensitive (ciprofloxacin minimum inhibitory concentration, MIC < 0.5 micro g/ml) and 10 ciprofloxacin-resistant (MIC 16 to 32 micro g/ml) presumptive C. jejuni were further characterized and evaluated for their inhibition by natural orange oil fractions. Partial species identification was performed by using a hippuricase gene-based polymerase chain reaction (PCR) assay. One of the isolates appeared to be atypical and failed to hydrolyze hippurate. Of the ciprofloxacin-resistant C. jejuni isolates tested, six were found to have their quinolone resistance determined by a C --> T mutation in codon 86 of gyrA. Both groups of ciprofloxacin-sensitive and -resistant C. jejuni isolates were most susceptible to cold-pressed terpeneless Valencia orange oil (C4) which yielded inhibition zones from 44.0 +/- 1.4 to 80 +/- 0.0 mm. Less inhibitory responses were recorded for 5-fold concentrated Valencia orange oil (C3) and distilled d-limonene (C7) which exerted similar effects on both ciprofloxacin-sensitive and -resistant C. jejuni isolates. In general, ciprofloxacin-resistant and -sensitive C. jejuni isolates were equally susceptible to the respective orange oil fractions.
共有 10 株环丙沙星敏感（环丙沙星最低抑菌浓度，MIC < 0.5 微克/毫升）和 10 株环丙沙星耐药（MIC 16 至 32 微克/毫升）的推定空肠弯曲菌进一步进行了特征描述，并评估了天然橙油馏分对其的抑制作用。部分种属鉴定是通过 hippuricase 基因基于聚合酶链反应（PCR）检测完成的。其中一个分离株似乎是非典型的，未能水解马尿酸。在测试的环丙沙星耐药空肠弯曲菌分离株中，有 6 株被发现其喹诺酮耐药性是由 gyrA 密码子 86 处的 C --> T 突变决定的。两组环丙沙星敏感和耐药空肠弯曲菌分离株对冷榨无萜橙油（C4）最敏感，其抑菌圈从 44.0 +/- 1.4 至 80 +/- 0.0 毫米不等。5 倍浓缩橙油（C3）和蒸馏 d-柠檬烯（C7）的抑制反应较小，对环丙沙星敏感和耐药空肠弯曲菌分离株均有类似的作用。总的来说，环丙沙星耐药和敏感的空肠弯曲菌分离株对各自的橙油馏分同样敏感。
